{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:01:58Z","timestamp":1790694118810,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Ameloblastoma is a benign odontogenic tumor characterized by locally infiltrative growth and a high recurrence rate despite minimal cytological atypia. Histologically, tumor budding\u2014small epithelial projections arising from tumor nests\u2014is frequently observed and has been suggested to be associated with recurrence; however, its molecular basis and pathological significance remain unclear. This study aimed to clarify the molecular characteristics of tumor budding, its relationship with surrounding bone remodeling, and its possible contribution to tumor recurrence. Immunohistochemical analyses revealed that HuR and \u0394Np63 were co\u2010expressed in budding areas of ameloblastoma. In vitro experiments using the AM\u20101 ameloblastoma cell line demonstrated that HuR binds to \u0394Np63 mRNA and stabilizes its expression. Genetic knockout of \u0394Np63 increased E\u2010cadherin expression, consistent with the reduced E\u2010cadherin expression observed immunohistochemically in \u0394Np63\u2010positive budding areas. Histological examination of serial sections revealed bone remodeling at the tumor front, with newly formed bone surrounding tumor nests together with areas of bone resorption. These findings suggest that alterations in HuR, \u0394Np63, and E\u2010cadherin are associated with tumor budding and reduced epithelial cell\u2013cell adhesion. Accompanying bone remodeling may complicate tumor removal and contribute to recurrence.<\/jats:p>","DOI":"10.1111\/pin.70178","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T14:14:51Z","timestamp":1789568091000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["HuR\u2013\u0394Np63 Regulation and E\u2010Cadherin Expression in Tumor Budding and Bone Remodeling of Ameloblastoma"],"prefix":"10.1111","volume":"76","author":[{"given":"Saki","family":"Shinohara","sequence":"first","affiliation":[{"name":"Department of Vascular Biology and Molecular Pathology, Graduate School of Dental Medicine Hokkaido University Sapporo Japan"},{"name":"Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine and Graduate School of Dental Medicine Hokkaido University Sapporo 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