{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T08:33:31Z","timestamp":1740126811384,"version":"3.37.3"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,4,9]],"date-time":"2015-04-09T00:00:00Z","timestamp":1428537600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007112","name":"University of Cape Town","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100007112","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Oppenheimer Fund (University of Oxford)"},{"name":"Equity Development Program at UCT"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biol J"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Anthropogenic activities have resulted in cyanide (<jats:styled-content style=\"fixed-case\">CN<\/jats:styled-content>) contamination of both soil and water in many areas of the globe. While plants possess a detoxification pathway that serves to degrade endogenously generated <jats:styled-content style=\"fixed-case\">CN<\/jats:styled-content>, this system is readily overwhelmed, limiting the use of plants in bioremediation. Genetic engineering of additional <jats:styled-content style=\"fixed-case\">CN<\/jats:styled-content> degradation pathways in plants is one potential strategy to increase their tolerance to <jats:styled-content style=\"fixed-case\">CN<\/jats:styled-content>. Here we show that heterologous expression of microbial nitrilase enzymes targeted to the mitochondria increases <jats:styled-content style=\"fixed-case\">CN<\/jats:styled-content> tolerance in <jats:italic>Arabidopsis<\/jats:italic>. Root length in seedlings expressing either a <jats:styled-content style=\"fixed-case\">CN<\/jats:styled-content> dihydratase from <jats:italic>Bacillus pumilis<\/jats:italic> or a <jats:styled-content style=\"fixed-case\">CN<\/jats:styled-content> hydratase from <jats:italic>Neurospora crassa<\/jats:italic> was increased by 45% relative in wild\u2010type plants in the presence of 50\u00a0\u03bc<jats:sc>m <\/jats:sc><jats:styled-content style=\"fixed-case\">KCN<\/jats:styled-content>. We also demonstrate that in contrast to its strong inhibitory effects on seedling establishment, seed germination of the Col\u20100 ecotype of <jats:italic>Arabidopsis<\/jats:italic> is unaffected by <jats:styled-content style=\"fixed-case\">CN<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/plb.12323","type":"journal-article","created":{"date-parts":[[2015,2,25]],"date-time":"2015-02-25T06:39:07Z","timestamp":1424846347000},"page":"922-926","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Heterologous expression of mitochondria\u2010targeted microbial nitrilase enzymes increases cyanide tolerance in <i>Arabidopsis<\/i>"],"prefix":"10.1111","volume":"17","author":[{"given":"E.","family":"Molojwane","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology University of Cape Town Rondebosch South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Adams","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology University of Cape Town Rondebosch South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. J.","family":"Sweetlove","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences University of Oxford Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. A.","family":"Ingle","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology University of Cape Town Rondebosch South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,4,9]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00709654"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-008-1559-2"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-005-0116-9"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0109853"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2007.04.007"},{"key":"e_1_2_5_7_1","first-page":"616","volume-title":"Cyanide in water and soil: chemistry, risk and management","author":"Dzombak D.A.","year":"2006"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3040.2010.02136.x"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1356"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.1996.tb00543.x"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1380077"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envexpbot.2014.09.004"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/jexbot\/51.346.865"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.19.6105-6112.1993"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/mp\/sst110"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-005-6217-9"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2009.04060.x"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.52.1.23"},{"key":"e_1_2_5_19_1","first-page":"128","article-title":"Feasibility of cyanide elimination using plants","volume":"3","author":"Trapp S.","year":"2003","journal-title":"The European Journal of Mineral Processing and Environmental Protection"},{"key":"e_1_2_5_20_1","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/S0021-9258(19)78190-3","article-title":"Inhibition of ribulose diphosphate carboxylase by cyanide: inactive ternary complex of enzyme, ribulose diphosphate, and cyanide","volume":"244","author":"Wishnick M.","year":"1969","journal-title":"Journal of Biological Chemistry"}],"container-title":["Plant Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fplb.12323","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/plb.12323","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T21:07:48Z","timestamp":1696021668000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/plb.12323"}},"subtitle":[],"editor":[{"given":"R.","family":"Mendel","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2015,4,9]]},"references-count":19,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,7]]}},"alternative-id":["10.1111\/plb.12323"],"URL":"https:\/\/doi.org\/10.1111\/plb.12323","archive":["Portico"],"relation":{},"ISSN":["1435-8603","1438-8677"],"issn-type":[{"type":"print","value":"1435-8603"},{"type":"electronic","value":"1438-8677"}],"subject":[],"published":{"date-parts":[[2015,4,9]]},"assertion":[{"value":"2014-11-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-02-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-04-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}