{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T07:20:09Z","timestamp":1696231209122},"reference-count":21,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,9,13]],"date-time":"2015-09-13T00:00:00Z","timestamp":1442102400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31370349"],"award-info":[{"award-number":["31370349"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biol J"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The low resolution of chromosome\u2010based Fluorescence <jats:italic>in\u00a0situ<\/jats:italic> hybridisation (<jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content>) mapping is primarily due to the structure of the plant cell wall and cytoplasm and the compactness of regular chromosomes, which represent a significant obstacle to <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content>. In order to improve spatial resolution and signal detection sensitivity, we provide a reproducible method to generate high\u2010quality extended chromosomes that are ~13 times as long as their pachytene counterparts. We demonstrate that proteinase K used in this procedure is crucial for stretching pachytene chromosomes of <jats:italic>Brassica oleracea<\/jats:italic> in the context of a modified Carnoy's <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> fixative (6:1:3, ethanol:chloroform:acetic acid). The quality of super\u2010stretched chromosomes was assessed in several <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> experiments. <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> signals from both repetitive 5S <jats:styled-content style=\"fixed-case\">rDNA<\/jats:styled-content> and single\u2010copy <jats:italic><jats:styled-content style=\"fixed-case\">ARC<\/jats:styled-content>1<\/jats:italic> on super\u2010stretched chromosomes are brighter than those on other different types of chromosome due to enhanced accessibility to targets on stretched pachytene chromosomes. In conclusion, the resulting extended chromosomes are suitable for <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> mapping for repetitive <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> sequences and the localisation of a single\u2010copy locus, and <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> performed on super\u2010stretched chromosomes can achieve significantly higher sensitivity and spatial resolution than other chromosome\u2010based <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> mapping techniques.<\/jats:p>","DOI":"10.1111\/plb.12384","type":"journal-article","created":{"date-parts":[[2015,8,27]],"date-time":"2015-08-27T15:27:29Z","timestamp":1440689249000},"page":"325-331","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Improvement of high\u2010resolution fluorescence <i>in\u00a0situ<\/i> hybridisation mapping on chromosomes of <i>Brassica oleracea<\/i> var<i>. capitata<\/i>"],"prefix":"10.1111","volume":"18","author":[{"given":"K.","family":"Yang","sequence":"first","affiliation":[{"name":"College of Agronomy and Biotechnology Southwest University Chongqing China"},{"name":"Engineering Research Center of South Upland Agriculture Ministry of Education Southwest University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Agronomy and Biotechnology Southwest University Chongqing China"},{"name":"Engineering Research Center of South Upland Agriculture Ministry of Education Southwest University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Converse","sequence":"additional","affiliation":[{"name":"Cincinnati State\u00a0Technical and Community College Cincinnati OH 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Master's Thesis Southwest University China."},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-011-1086-y"}],"container-title":["Plant Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fplb.12384","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/plb.12384","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T09:17:00Z","timestamp":1696151820000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/plb.12384"}},"subtitle":[],"editor":[{"given":"J.","family":"Keurentjes","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2015,9,13]]},"references-count":21,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,3]]}},"alternative-id":["10.1111\/plb.12384"],"URL":"https:\/\/doi.org\/10.1111\/plb.12384","archive":["Portico"],"relation":{},"ISSN":["1435-8603","1438-8677"],"issn-type":[{"value":"1435-8603","type":"print"},{"value":"1438-8677","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9,13]]},"assertion":[{"value":"2015-07-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-08-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-09-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}