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Tomato (<jats:italic>Solanum lycopersicum<\/jats:italic> cv Micro\u2010Tom) roots were exposed directly or gradually to low <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> through step\u2010wise changes in <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> over periods ranging from 4 to 24\u00a0h. Roots exposed gradually to <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> 4.5 grew even less than those exposed directly, indicating a plant\u2010coordinated response. Direct exposure to <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> 4.0 suppressed root growth and caused high cell mortality, in contrast to roots exposed gradually, in which growth remained inhibited but cell viability was maintained. Total class <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content> peroxidase activity increased significantly in all low <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> treatments, but was not correlated with the observed differential responses. Use of the enzyme inhibitors salicylhydroxamic acid (<jats:styled-content style=\"fixed-case\">SHAM<\/jats:styled-content>) or diphenyleneiodonium chloride (<jats:styled-content style=\"fixed-case\">DPI<\/jats:styled-content>) suggest that peroxidase and, to a lesser extent, <jats:styled-content style=\"fixed-case\">NADPH<\/jats:styled-content> oxidase were required to prevent or reduce injury in all low <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> treatments. However, a role for other enzymes, such as the alternative oxidase is also possible. The results with <jats:styled-content style=\"fixed-case\">SHAM<\/jats:styled-content>, but not <jats:styled-content style=\"fixed-case\">DPI<\/jats:styled-content>, were confirmed in tobacco <jats:styled-content style=\"fixed-case\">BY<\/jats:styled-content>\u20102 cells. Our results indicate that root growth inhibition from low <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> can be part of an active plant response, and suggest that peroxidases may have a critical early role in reducing loss of cell viability and in the observed root growth constraint.<\/jats:p>","DOI":"10.1111\/plb.12443","type":"journal-article","created":{"date-parts":[[2016,2,19]],"date-time":"2016-02-19T02:53:05Z","timestamp":1455850385000},"page":"658-668","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Root growth restraint can be an acclimatory response to low <scp>pH<\/scp> and is associated with reduced cell mortality: a possible role of class <scp>III<\/scp> peroxidases and <scp>NADPH<\/scp> oxidases"],"prefix":"10.1111","volume":"18","author":[{"given":"J. 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