{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T06:57:57Z","timestamp":1770447477393,"version":"3.49.0"},"reference-count":48,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,7,12]],"date-time":"2016-07-12T00:00:00Z","timestamp":1468281600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004695","name":"Inyuvesi Yakwazulu-Natali","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004695","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biol J"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>\n<jats:list list-type=\"bullet\">\n\n<jats:list-item><jats:p><jats:italic>Hypoxis hemerocallidea<\/jats:italic> is a highly utilized medicinal plant in South Africa. Its cultivation has received considerable attention in order to meet the high demand. High levels of cadmium (Cd) and aluminum (Al) in <jats:italic>H.\u00a0hemerocallidea<\/jats:italic> plants sold in traditional medicinal markets was previously reported.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>The present study used an <jats:italic>in\u00a0vitro<\/jats:italic> propagation model to investigate the uptake of Cd and Al by <jats:italic>H.\u00a0hemerocallidea<\/jats:italic> and their effect on plant growth, elemental uptake and some stress\u2010induced responses such as pigment, malondialdehyde (MDA), proline content and ultrastructural changes.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Shoot and root growth of plantlets exposed to Cd, Cd:Al and high concentrations of Al was significantly reduced. Highest concentrations of Cd accumulated in the corms of Cd\u2010treated plantlets while highest Al concentrations occurred in the leaves and roots. There was higher accumulation of Cd and Al when applied singularly compared to the Cd:Al combination treatments. Cd and Al also reduced accumulation of trace elements in micropropagted <jats:italic>H<\/jats:italic>.\u00a0<jats:italic>hemerocallidea<\/jats:italic> with lowest concentrations in the Cd:Al combination treatments. Exposure to Cd, Al and Cd:Al significantly reduced the level of chlorophyll but increased the levels of carotenoids, MDA and proline. Ultrastructural changes were also observed in <jats:italic>H.\u00a0hemerocallidea<\/jats:italic> exposed to Cd and Al.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>All these factors contributed to the inhibition of plant growth and could potentially affect the ability of this important medicinal plant to synthesize bioactive compounds. It is thus necessary to understand heavy metal stress\u2010induced responses in this highly valued medicinal plant to ensure a high quality product for the consumer.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><\/jats:sec>","DOI":"10.1111\/plb.12480","type":"journal-article","created":{"date-parts":[[2016,6,16]],"date-time":"2016-06-16T08:46:33Z","timestamp":1466066793000},"page":"805-815","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Investigating the effect of cadmium and aluminium on growth and stress\u2010induced responses in the micropropagated medicinal plant <i>Hypoxis hemerocallidea<\/i>"],"prefix":"10.1111","volume":"18","author":[{"given":"A.","family":"Okem","sequence":"first","affiliation":[{"name":"Research Centre for Plant Growth and Development School of Life Sciences University of KwaZulu\u2010Natal Pietermaritzburg Scottsville South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Moyo","sequence":"additional","affiliation":[{"name":"Research Centre for Plant Growth and Development School of Life Sciences University of KwaZulu\u2010Natal Pietermaritzburg Scottsville South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. 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