{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T14:21:26Z","timestamp":1792074086799,"version":"4.4.0"},"reference-count":73,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,9,22]],"date-time":"2018-09-22T00:00:00Z","timestamp":1537574400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biol J"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n<jats:list list-type=\"bullet\">\n\n<jats:list-item><jats:p>Aluminum (Al) and proton (H<jats:sup>+<\/jats:sup>) ions are major acid soil stress factors deleteriously affecting plant root growth and crop yield. In our preliminary studies, cotton (<jats:italic>Gossypium hirsutum<\/jats:italic> L.) seedlings displayed very sensitive phenotypes to Al and H<jats:sup>+<\/jats:sup> rhizotoxicities.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Based on previous <jats:italic>Arabidopsis<\/jats:italic> results, we aimed to characterise the Al stress responsive Sensitive to Proton rhizotoxicity 1 (<jats:italic>GhSTOP1<\/jats:italic>) transcription system in cotton using RNAi\u2010mediated down\u2010regulation. With the help of seed embryo apex explants, we developed transgenic cotton plants overexpressing a <jats:italic>GhSTOP1\u2010<\/jats:italic>RNAi cassette with NPTII selection. Kanamycin\u2010tolerant T1 seedlings were further considered for Al and H<jats:sup>+<\/jats:sup> stress tolerance studies.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Down\u2010regulation of the <jats:italic>GhSTOP1<\/jats:italic> increased sensitivity to Al and proton rhizotoxicities, and root growth was significantly reduced in RNAi lines. The expression profile of <jats:italic>GhALMT1<\/jats:italic> (<jats:italic>Aluminum\u2010activated Malate Transporter 1<\/jats:italic>)<jats:italic>, GhMATE<\/jats:italic> (<jats:italic>Multidrug and Toxic Compound Extrusion<\/jats:italic>), <jats:italic>GhALS3<\/jats:italic> (<jats:italic>Aluminium Sensitive 3<\/jats:italic>) and key genes involved in the GABA shunt were down\u2010regulated in the transgenic RNAi lines. Additionally, the lateral root initiation process was delayed and expression of <jats:italic>GhNAC1<\/jats:italic>, which is involved in lateral roots, was also suppressed in transgenic lines. Besides, overexpression of <jats:italic>GhSTOP1<\/jats:italic> in <jats:italic>Arabidopsis<\/jats:italic> accelerated root growth and <jats:italic>AtMATE<\/jats:italic> and <jats:italic>AtALMT1<\/jats:italic> expression under Al stress conditions.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>These analyses indicate that <jats:italic>GhSTOP1<\/jats:italic> is essential for the expression of several genes which are necessary for acid soil tolerance mechanisms and lateral root initiation.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/plb.12895","type":"journal-article","created":{"date-parts":[[2018,8,11]],"date-time":"2018-08-11T06:49:25Z","timestamp":1533970165000},"page":"35-44","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":58,"title":["<i>GhSTOP1<\/i>, a C2H2 type zinc finger transcription factor is essential for aluminum and proton stress tolerance and lateral root initiation in cotton"],"prefix":"10.1111","volume":"21","author":[{"given":"A.","family":"Kundu","sequence":"first","affiliation":[{"name":"Department of Life Sciences Presidency University Kolkata West Bengal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Life Sciences Presidency University Kolkata West Bengal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Basu","sequence":"additional","affiliation":[{"name":"Department of Life Sciences Presidency University Kolkata West Bengal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Applied Biological Sciences Gifu University Gifu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Applied Biological Sciences Gifu University Gifu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Koyama","sequence":"additional","affiliation":[{"name":"Department of Applied Biological Sciences Gifu University Gifu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4338-3027","authenticated-orcid":false,"given":"M.","family":"Ganesan","sequence":"additional","affiliation":[{"name":"Department of Life Sciences Presidency University Kolkata West Bengal India"},{"name":"Department of Applied Biological Sciences Gifu University Gifu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.111.1.9"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0098-8472(02)00013-8"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00009580"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1360-1385(03)00051-7"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.112.199778"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.12266"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcw126"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313x.1998.00343.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406258101"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1201\/9780824746568.ch1"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-5-39"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0186084"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.12258"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/15592324.2015.1131371"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5318.1566"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3844\/ajbbsp.2009.63.74"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.12887"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2229-13-134"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020835304842"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1897\/08-617.1"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.75.4.1017"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02374262"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0602868103"},{"key":"e_1_2_7_25_1","first-page":"258","article-title":"Identification of STOP1\u2010like proteins associated with aluminum tolerance in sweet Sorghum (Sorghum bicolor L.). 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