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Presence of excess NaCl in the soil is one of the main causes of reduced crop productivity worldwide. The present investigation examines the role of exogenous Mel and endogenous H<jats:sub>2<\/jats:sub>S in tomato seedlings grown under NaCl stress.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Effect of 30\u00a0\u00b5<jats:sc>m<\/jats:sc> Mel on endogenous synthesis of H<jats:sub>2<\/jats:sub>S was examined in roots of NaCl\u2010stressed (200\u00a0m<jats:sc>m<\/jats:sc>) tomato seedlings. Also, the impact of treatments on the oxidative stress markers, transport of K<jats:sup>+<\/jats:sup> and Na<jats:sup>+<\/jats:sup>, and activity of H<jats:sup>+<\/jats:sup>\u2010ATPase and antioxidant enzymes was assessed.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Results show that NaCl\u2010stressed seedlings supplemented with 30\u00a0\u00b5<jats:sc>m<\/jats:sc> Mel had increased levels of endogenous H<jats:sub>2<\/jats:sub>S through enhanced L\u2010cysteine desulfhydrase activity. Mel in association with H<jats:sub>2<\/jats:sub>S overcame the deleterious effect of NaCl and induced retention of K<jats:sup>+<\/jats:sup> that maintained a higher K<jats:sup>+<\/jats:sup>\/Na<jats:sup>+<\/jats:sup> ratio. Use of plasma membrane inhibitors and an H<jats:sub>2<\/jats:sub>S scavenger revealed that Mel\u2010induced regulation of K<jats:sup>+<\/jats:sup>\/Na<jats:sup>+<\/jats:sup> homeostasis in NaCl\u2010stressed seedling roots operates through endogenous H<jats:sub>2<\/jats:sub>S signalling. Synergistic effects of Mel and H<jats:sub>2<\/jats:sub>S also reduced the generation of ROS and oxidative destruction through the enhanced activity of antioxidant enzymes.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Thus, it is suggested that the protective function of Mel against NaCl stress operates through an endogenous H<jats:sub>2<\/jats:sub>S\u2010dependent pathway, wherein H<jats:sup>+<\/jats:sup>\u2010ATPase\u2010energized secondary active transport regulates K<jats:sup>+<\/jats:sup>\/Na<jats:sup>+<\/jats:sup> homeostasis.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/plb.13296","type":"journal-article","created":{"date-parts":[[2021,7,15]],"date-time":"2021-07-15T11:11:23Z","timestamp":1626347483000},"page":"797-805","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["Exogenous melatonin\u2010mediated regulation of K<sup>+<\/sup>\/Na<sup>+<\/sup> transport, H<sup>+<\/sup>\u2010ATPase activity and enzymatic antioxidative defence operate through endogenous hydrogen sulphide signalling in NaCl\u2010stressed tomato seedling roots"],"prefix":"10.1111","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2840-4608","authenticated-orcid":false,"given":"M. H.","family":"Siddiqui","sequence":"first","affiliation":[{"name":"Department of Botany and Microbiology College of Science King Saud University Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3039-0305","authenticated-orcid":false,"given":"M. N.","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Biology College of Haql University of Tabuk Tabuk Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"Department of Botany Jangipur College University of Kalyani Kalyani West Bengal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. A.","family":"Basahi","sequence":"additional","affiliation":[{"name":"Department of Biology College of Haql University of Tabuk Tabuk Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Alamri","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology College of Science King Saud University Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. A.","family":"Al\u2011Amri","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology College of Science King Saud University Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Q. D.","family":"Alsubaie","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology College of Science King Saud University Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. 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