{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T01:44:25Z","timestamp":1784598265180,"version":"3.55.0"},"reference-count":54,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,12,17]],"date-time":"2021-12-17T00:00:00Z","timestamp":1639699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31960581"],"award-info":[{"award-number":["31960581"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biol J"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n<jats:list list-type=\"bullet\">\n\n<jats:list-item><jats:p>Iron (Fe) is an essential element for plant growth and development. Fe deficiency can trigger leaf chlorosis and reduce fruit yield. Therefore, it is necessary to explore transcription factors in response to Fe deficiency stress.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>A total of 29 <jats:italic>MhR2R3\u2010MYB<\/jats:italic> transcription factors were identified based on the transcriptome of <jats:italic>Malus halliana<\/jats:italic> under Fe deficiency stress. A comprehensive analysis of physical and chemical properties, gene structures, conserved motif composition, evolutionary relationship and chromosome distribution was performed. Subsequently, based on the transcriptome, 14 genes with the most significant expression under Fe deficiency stress were screened for qRT\u2010PCR verification. Among them,the functional characteristics of <jats:italic>MhR2R3\u2010MYB4<\/jats:italic> (MD05G1089600) were further studied in <jats:italic>Arabidopsis thaliana<\/jats:italic>.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Expression of 13 out of these 14 genes was upregulated, only one was downregulated. Maximum upregulation of <jats:italic>MhR2R3\u2010MYB4<\/jats:italic> under Fe deficiency was 36.39\u2010fold and 58.21\u2010fold compared with day 0 in leaves and roots, respectively. Overexpression of <jats:italic>MhR2R3\u2010MYB4<\/jats:italic> enhanced tolerance to Fe deficiency in <jats:italic>A. thaliana<\/jats:italic> and led to multiple biochemical changes: transgenic lines have higher chl <jats:italic>a<\/jats:italic>, chl <jats:italic>b<\/jats:italic> and Fe<jats:sup>2+<\/jats:sup> content, higher enzyme activity (SOD, POD, CAT and FCR) and lower chlorosis than the wild type in Fe deficiency conditions.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>We suggest that <jats:italic>MhR2R3\u2010MYB4<\/jats:italic> plays an important part in Fe deficiency stress, which may contribute to improve Fe deficiency tolerance of apple in future.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/plb.13373","type":"journal-article","created":{"date-parts":[[2021,12,18]],"date-time":"2021-12-18T07:35:15Z","timestamp":1639812915000},"page":"344-355","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Identification of <i>Malus halliana R2R3\u2010MYB<\/i> gene family under iron deficiency stress and functional characteristics of <i>MhR2R3\u2010MYB4<\/i> in <i>Arabidopsis thaliana<\/i>"],"prefix":"10.1111","volume":"24","author":[{"given":"Z.\u2010X.","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Horticulture Gansu Agricultural University Lanzhou 730070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Horticulture Gansu Agricultural University Lanzhou 730070 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