{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T20:22:48Z","timestamp":1775593368862,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,6,30]],"date-time":"2022-06-30T00:00:00Z","timestamp":1656547200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2022,6,30]],"date-time":"2022-06-30T00:00:00Z","timestamp":1656547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001117","name":"AW Howard Memorial Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001117","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001779","name":"Monash University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001779","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["DP210103010"],"award-info":[{"award-number":["DP210103010"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biol J"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n<jats:list list-type=\"bullet\">\n\n<jats:list-item><jats:p>Domesticated sorghum (<jats:italic>Sorghum bicolor<\/jats:italic> [L.] Moench subsp. <jats:italic>bicolor<\/jats:italic>) diverts significant amounts of nitrogen away from primary metabolism to the synthesis of cyanogenic glucosides (CNglc) \u2013 specialized metabolites that release toxic hydrogen cyanide (HCN). Our aim was to identify the point in the genus <jats:italic>Sorghum<\/jats:italic> Moench at which plants gained the ability to maintain hazardous concentrations of cyanogenic glucosides in their leaves into maturity (HCN potential &gt;0.4\u2009mg\u2009g<jats:sup>\u22121<\/jats:sup>). This ability occurs in domesticated sorghum (in the subgenus <jats:italic>Eusorghum<\/jats:italic>), but not in wild taxa in other <jats:italic>Sorghum<\/jats:italic> subgenera.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Eight accessions from the subgenus <jats:italic>Eusorghum<\/jats:italic> were grown in a common garden: an improved sorghum line, five sorghum landraces, the crop's wild progenitor (<jats:italic>S. bicolor<\/jats:italic> subsp. <jats:italic>verticilliflorum<\/jats:italic> [Steud.] de Wet ex Wiersema &amp; J. Dahlb.) and wild <jats:italic>Sorghum propinquum<\/jats:italic> (Kunth) Hitchc. HCN potential was measured in plants (n\u2009=\u200980) at the three\u2010leaf stage and at 6\u2009weeks old.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>All study accessions, including the wild taxa, had hazardous CNglc concentrations in the leaves at both the three\u2010leaf stage (mean HCN potentials &gt;\u2009=\u20092.5\u2009mg\u2009g<jats:sup>\u22121<\/jats:sup>) and at 6\u2009weeks old (mean HCN potentials &gt;\u2009=\u20090.68\u2009mg\u2009g<jats:sup>\u22121<\/jats:sup>), greatly contrasting the much lower mature leaf HCN potentials previously found in wild <jats:italic>Sorghum<\/jats:italic> taxa outside subgenus <jats:italic>Eusorghum<\/jats:italic> (generally &lt;=\u20090.01\u2009mg\u2009g<jats:sup>\u22121<\/jats:sup>).<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Our results suggest that the ability to maintain hazardous leaf HCN potentials into maturity might have arisen during the divergence of <jats:italic>Eusorghum<\/jats:italic> from other <jats:italic>Sorghum<\/jats:italic> subgenera, rather than during the speciation or domestication of <jats:italic>S. bicolor<\/jats:italic>, and highlights the value of utilizing <jats:italic>Sorghum<\/jats:italic> taxa outside <jats:italic>Eusorghum<\/jats:italic> in efforts to improve the crop safety of sorghum.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/plb.13447","type":"journal-article","created":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T17:34:00Z","timestamp":1655832840000},"page":"1084-1088","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Regulation of cyanogenic glucosides in wild and domesticated <i>Eusorghum<\/i> taxa"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2690-6188","authenticated-orcid":false,"given":"H.","family":"Myrans","sequence":"first","affiliation":[{"name":"School of Biological Sciences Monash University  Clayton Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4756-0411","authenticated-orcid":false,"given":"R. 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