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T\u2010DNA recessive mutant, which exhibits a dwarf and chlorotic phenotype. Functional studies of the T\u2010DNA tagged gene were conducted, including phylogenetic analysis, expression and metabolomic analyses, and generation of CRISPR\/Cas9 knockout lines.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>The cloning of T\u2010DNA flanking genomic sequences and a co\u2010segregation analysis found the <jats:italic>pad<\/jats:italic> phenotype was caused by a T\u2010DNA insertion disrupting the tomato homologue of the <jats:italic>Arabidopsis SUPPRESSOR OF SALICYLIC ACID INSENSITIVITY 2<\/jats:italic> (<jats:italic>SlSSI2<\/jats:italic>), encoding a plastid localized isoform of SACPD. The phenotype of CRISPR\/Cas9 <jats:italic>SlSSI2<\/jats:italic> knockout lines confirmed that the morphological abnormalities in <jats:italic>pad<\/jats:italic> plants were due to <jats:italic>SlSSI2<\/jats:italic> loss of function. Functional, metabolomic and expression analyses proved that <jats:italic>SlSSI2<\/jats:italic> disruption causes deficiencies in 18:1 fatty acid desaturation and leads to diminished jasmonic acid (JA) content and increased salicylic acid (SA) levels.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Overall, these results proved that SSI2 plays a crucial role in the regulation of polyunsaturated fatty acid profiles in tomato, and revealed that <jats:italic>SlSSI2<\/jats:italic> loss of function results in an inhibited JA\u2010responsive signalling pathway and a constitutively activated SA\u2010mediated defence signalling response. This study lays the foundation for further research on tomato SACPDs and their role in plant performance and fitness.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/plb.13591","type":"journal-article","created":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T21:31:04Z","timestamp":1700602264000},"page":"106-116","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Loss of function mutations at the tomato <i>SSI2<\/i> locus impair plant growth and development by altering the fatty acid desaturation pathway"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2894-3409","authenticated-orcid":false,"given":"A. 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