{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T12:03:32Z","timestamp":1771243412642,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T00:00:00Z","timestamp":1761177600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T00:00:00Z","timestamp":1761177600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biol J"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:list list-type=\"bullet\">\n                      <jats:list-item>\n                        <jats:p>\n                          \u2018Wuzihong 1\u20107\u2019 is a novel seedless low\u2010acid sweet orange mutant developed through\n                          <jats:sup>60<\/jats:sup>\n                          Co\u2010\u03b3 irradiation of \u2018Youzihong 1\u20107\u2019. This study investigates its multi\u2010level seedlessness mechanisms through comparative analysis with the parental line.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Experiments included modified Carbol\u2010fuchsin staining for pollen meiosis; SEM analysis of pollen morphology\/germination; paraffin sectioning for embryo sac development; and ISSR markers for genomic variation detection.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Abnormal pollen meiosis resulted in 10.1% viability (vs. 85.7% in control), 89.9% embryo sac development arrest, predominantly at mononuclear (38.2%) and binuclear (42.7%) stages. Post\u2010fertilization embryos showed 71.3% arrest at globular stage, and three specific ISSR markers (UBC\u2010813, UBC\u2010834, and UBC\u2010835) confirmed that \u2018Wuzihong 1\u20107\u2019 is a genetically stable variant.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Seedlessness in \u2018Wuzihong 1\u20107\u2019 is mainly caused via three primary mechanisms: male sterility (meiotic defects), female gametophyte abortion, and post\u2010pollination developmental barriers. The identified ISSR markers will provide molecular tools for seedless trait selection. This multi\u2010omics analysis advances understanding of radiation\u2010induced seedlessness and facilitates targeted breeding of seedless citrus varieties.<\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/plb.70124","type":"journal-article","created":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T04:25:25Z","timestamp":1761279925000},"page":"116-127","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated cytological and molecular analysis reveals the seedless mechanism in a\n                    <scp>\n                      <sup>60<\/sup>\n                      Co\n                    <\/scp>\n                    \u2010\u03b3 induced low\u2010acid sweet orange mutant \u2018Wuzihong 1\u20107\u2019 (\n                    <i>Citrus sinensis<\/i>\n                    Osbeck)"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8400-337X","authenticated-orcid":false,"given":"Z.","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory for Conservation and Utilization of Subtropical Agro\u2010Bioresources\/Guangdong, Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables\/Key Laboratory of South China, Horticultural Crop Biology and Germplasm Enhancement, College of Horticulture South China Agricultural University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Pan","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Conservation and Utilization of Subtropical Agro\u2010Bioresources\/Guangdong, Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables\/Key Laboratory of South China, Horticultural Crop Biology and Germplasm Enhancement, College of Horticulture South China Agricultural 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