{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T18:08:29Z","timestamp":1790186909155,"version":"4.1.0"},"reference-count":76,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["245783\/2012\u20101"],"award-info":[{"award-number":["245783\/2012\u20101"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["245777\/2012\u20101"],"award-info":[{"award-number":["245777\/2012\u20101"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["302426\/2023\u20100"],"award-info":[{"award-number":["302426\/2023\u20100"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biol J"],"abstract":"Abstract<\/jats:title>\n \n \n \n \n Orchid seeds primarily contain lipid reserves, yet details of their deposition, in quantity and quality, remain unknown. Deposition begins as carbohydrates, which are eventually converted into fatty acids during seed development. This research aimed to dissect the pattern of lipid deposition in seeds of\n Dactylorhiza fuchsii<\/jats:italic>\n and\n Disa uniflora<\/jats:italic>\n , from pollination to seed storage, including at five stages of seed maturation, germination capacity and relationships among fatty acids (FAs).\n <\/jats:p>\n <\/jats:list-item>\n \n \n The capsules were harvested, cleaned and measured. Extracted seeds were analysed using differential scanning calorimetry (DSC), moisture content determinations,\n in vitro<\/jats:italic>\n germination tests in three different media and seed viability. A portion of the seeds was dried to analyse lipid content and types via Gas Chromatography\u2013Mass Spectrometry (GC\u2013MS).\n <\/jats:p>\n <\/jats:list-item>\n \n \n While\n Dact. fuchsii<\/jats:italic>\n seeds showed a high percentage of dry matter at the end of maturation,\n Disa uniflora<\/jats:italic>\n had a lower one. Lipid accumulation profiles differed. The proportions of saturated and unsaturated FA varied, with\n Disa uniflora<\/jats:italic>\n having more saturated FA than\n Dact. fuchsii<\/jats:italic>\n . Seeds began to germinate before the midpoint of capsule maturation, and germination increased with maturation time in both species, with\n Disa uniflora<\/jats:italic>\n seeds reaching full germination at capsule dehiscence. Conversely,\n Dact. fuchsii<\/jats:italic>\n seeds did not germinate because the seed carapace may hinder germination. Nevertheless, at capsule maturity, seeds from both species were highly viable based on the tetrazolium test.\n <\/jats:p>\n <\/jats:list-item>\n \n Mature seeds of both species were desiccation\u2010tolerant (4.5% RH) and could be stored dry at 5\u2009\u00b0C for 3\u2009months without loss of viability, suggesting the possibility of long\u2010term seed banking for conservation.<\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>","DOI":"10.1111\/plb.70295","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T17:40:00Z","timestamp":1790185200000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Lipidomics of orchid seeds: from development to storage"],"prefix":"10.1111","author":[{"given":"C. C.","family":"Cust\u00f3dio","sequence":"first","affiliation":[{"name":"Agronomy College Unoeste Campus II Presidente Prudente Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0914-0686","authenticated-orcid":false,"given":"N. B.","family":"Machado\u2010Neto","sequence":"additional","affiliation":[{"name":"Agronomy College Unoeste Campus II Presidente Prudente Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Colville","sequence":"additional","affiliation":[{"name":"Royal Botanic Gardens, Kew Ardingly UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. T.","family":"Seaton","sequence":"additional","affiliation":[{"name":"Royal Botanic Gardens, Kew Ardingly UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. 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