{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T23:00:26Z","timestamp":1790118026564,"version":"4.0.1"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,5,28]],"date-time":"2013-05-28T00:00:00Z","timestamp":1369699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:p><jats:styled-content style=\"fixed-case\">MYB<\/jats:styled-content> transcription factors (<jats:styled-content style=\"fixed-case\">TFs<\/jats:styled-content>) involve in plant abiotic stress tolerance and response in various plant species. In this study, rapid amplification of cDNA ends (RACE)  was conducted to isolate the <jats:styled-content style=\"fixed-case\">R2R3\u2010MYB TF<\/jats:styled-content> gene <jats:italic><jats:styled-content style=\"fixed-case\">MdSIMYB1<\/jats:styled-content><\/jats:italic> from apples (<jats:italic>Malus<\/jats:italic>\u2009\u00d7\u2009<jats:italic>domestica<\/jats:italic>). The gene transcripts were abundant in the leaves, flowers and fruits, compared to other organs, and were induced by abiotic stresses and plant hormones. We observed the subcellular localization of an <jats:styled-content style=\"fixed-case\">MdSIMYB1\u2010GFP<\/jats:styled-content> fusion protein in the nucleus. Furthermore, the <jats:italic><jats:styled-content style=\"fixed-case\">MdSIMYB1<\/jats:styled-content><\/jats:italic> gene was introduced into the tobacco genome and ectopically expressed in transgenic lines. The results indicate that <jats:italic><jats:styled-content style=\"fixed-case\">MdSIMYB1<\/jats:styled-content><\/jats:italic> transgenic tobacco seed germination is insensitive to abscisic acid and <jats:styled-content style=\"fixed-case\">NaCl<\/jats:styled-content> treatment. Additionally, it was found that the ectopic expression of <jats:italic><jats:styled-content style=\"fixed-case\">MdSIMYB1<\/jats:styled-content><\/jats:italic> enhanced the tolerance of plants to high salinity, drought and cold tolerance by upregulating the stress\u2010responsive genes <jats:italic><jats:styled-content style=\"fixed-case\">NtDREB1A<\/jats:styled-content><\/jats:italic>, <jats:italic><jats:styled-content style=\"fixed-case\">NtERD10B<\/jats:styled-content><\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">NtERD10C<\/jats:styled-content><\/jats:italic>. Meanwhile, the transgenic tobacco exhibited robust root growth because of the enhanced expression of the auxin\u2010responsive genes <jats:italic><jats:styled-content style=\"fixed-case\">NtIAA4<\/jats:styled-content>.2<\/jats:italic>, <jats:italic><jats:styled-content style=\"fixed-case\">NtIAA4<\/jats:styled-content>.1<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">NtIAA2<\/jats:styled-content>.5<\/jats:italic> under stress conditions, which is conducive to stress tolerance. Finally, transgenic apple lines were obtained and tested. Transgenic apple lines that were overexpressing <jats:italic><jats:styled-content style=\"fixed-case\">MdSIMYB1<\/jats:styled-content><\/jats:italic> exhibited a higher tolerance to abiotic stress than the wild\u2010type control, but suppression of <jats:italic><jats:styled-content style=\"fixed-case\">MdSIMYB1<\/jats:styled-content><\/jats:italic> resulted in lower tolerance. Our results indicate that <jats:italic><jats:styled-content style=\"fixed-case\">MdSIMYB1<\/jats:styled-content><\/jats:italic> may be utilized as a target gene for enhancing stress tolerance in important crops.<\/jats:p>","DOI":"10.1111\/ppl.12069","type":"journal-article","created":{"date-parts":[[2013,5,6]],"date-time":"2013-05-06T13:30:41Z","timestamp":1367847041000},"page":"76-87","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":116,"title":["Overexpression of a <scp>R2R3 MYB<\/scp> gene <i><scp>MdSIMYB1<\/scp><\/i> increases tolerance to multiple stresses in transgenic tobacco and apples"],"prefix":"10.1111","volume":"150","author":[{"given":"Rong\u2010Kai","family":"Wang","sequence":"first","affiliation":[{"name":"National Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering Shandong Agricultural University  Tai\u2010An 271018 Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong\u2010Hui","family":"Cao","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering Shandong Agricultural University  Tai\u2010An 271018 Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Jin","family":"Hao","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering Shandong Agricultural University  Tai\u2010An 271018 Shandong 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