{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T21:52:26Z","timestamp":1787694746591,"version":"build-2784847793"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,9,13]],"date-time":"2013-09-13T00:00:00Z","timestamp":1379030400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:p>In this study, the short\u2010term and dynamic changes of the ethylene biosynthesis of Jonagold apple during and after application of controlled atmosphere (<jats:styled-content style=\"fixed-case\">CA<\/jats:styled-content>) storage conditions were quantified using a systems biology approach. Rapid responses to imposed temperature and atmospheric conditions were captured by continuous online photoacoustic ethylene measurements. Discrete destructive sampling was done to understand observed changes of ethylene biosynthesis at the transcriptional, translational and metabolic level. Application of the ethylene inhibitor 1\u2010methylcyclopropene (1\u2010<jats:styled-content style=\"fixed-case\">MCP<\/jats:styled-content>) allowed for the discrimination between ethylene\u2010mediated changes and ethylene\u2010independent changes related to the imposed conditions. Online ethylene measurements showed fast and slower responses during and after application of <jats:styled-content style=\"fixed-case\">CA<\/jats:styled-content> conditions. The changes in 1\u2010aminocyclopropane\u20101\u2010carboxylate synthase (<jats:styled-content style=\"fixed-case\">ACS<\/jats:styled-content>) activity were most correlated with changes in <jats:italic><jats:styled-content style=\"fixed-case\">ACS1<\/jats:styled-content><\/jats:italic> expression and regulated the cold\u2010induced increase in ethylene production during the early chilling phase. Transcription of <jats:italic><jats:styled-content style=\"fixed-case\">ACS3<\/jats:styled-content><\/jats:italic> was found ethylene independent and was triggered upon warming of <jats:styled-content style=\"fixed-case\">CA<\/jats:styled-content>\u2010stored apples. Increased expression of <jats:italic><jats:styled-content style=\"fixed-case\">ACO1<\/jats:styled-content><\/jats:italic> during shelf life led to a strong increase in 1\u2010aminocyclopropane\u20101\u2010carboxylate oxidase (<jats:styled-content style=\"fixed-case\">ACO<\/jats:styled-content>) activity, required for the exponential production of ethylene during system 2. Expression of <jats:italic><jats:styled-content style=\"fixed-case\">ACO2<\/jats:styled-content><\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">ACO3<\/jats:styled-content><\/jats:italic> was upregulated in 1\u2010<jats:styled-content style=\"fixed-case\">MCP<\/jats:styled-content>\u2010treated fruit showing a negative correlation with ethylene production. <jats:styled-content style=\"fixed-case\">ACO<\/jats:styled-content> activity never became rate limiting.<\/jats:p>","DOI":"10.1111\/ppl.12084","type":"journal-article","created":{"date-parts":[[2013,8,19]],"date-time":"2013-08-19T12:26:39Z","timestamp":1376915199000},"page":"161-173","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Dynamic changes of the ethylene biosynthesis in \u2018Jonagold\u2019 apple"],"prefix":"10.1111","volume":"150","author":[{"given":"Inge","family":"Bulens","sequence":"first","affiliation":[{"name":"Division of Mechatronics, Biostatistics and Sensors (MeBioS), Department of Biosystems (BIOSYST) Katholieke Universiteit Leuven Willem de Croylaan 42, bus 2428 B\u20103001 Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bram","family":"Van de Poel","sequence":"additional","affiliation":[{"name":"Division of Mechatronics, Biostatistics and Sensors (MeBioS), Department of Biosystems (BIOSYST) Katholieke Universiteit Leuven Willem de Croylaan 42, bus 2428 B\u20103001 Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maarten L. 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