{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T04:21:03Z","timestamp":1778905263957,"version":"3.51.4"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,9,14]],"date-time":"2013-09-14T00:00:00Z","timestamp":1379116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:p>A genotype \u2018Sujata\u2019 developed earlier at <jats:styled-content style=\"fixed-case\">CSIR\u2010CIMAP<\/jats:styled-content> from its parent \u2018Sampada\u2019 is considered to be the latex\u2010less variety of <jats:italic>Papaver somniferum<\/jats:italic>. These two genotypes are contrasting in terms of latex and stem strength. Earlier we have carried out microarray analysis to identify differentially expressing genes from the capsules of the two genotypes. In this study, the peduncles of the two genotypes were compared for the anatomy revealing less number of laticifers in the cortex and vascular bundles. One of the important cell wall\u2010related genes (for <jats:italic>laccase<\/jats:italic>) from the microarray analysis showing significantly higher expression in \u2018Sampada\u2019 capsule was taken up for further characterization in the peduncle here. It was functionally characterized through transient overexpression and <jats:styled-content style=\"fixed-case\">RNAi<\/jats:styled-content> suppression in \u2018Sujata\u2019 and \u2018Sampada\u2019. The increase in acid insoluble lignin and total lignin in overexpressed tissue of \u2018Sujata\u2019, and comparable decrease in suppressed tissue of \u2018Sampada\u2019, along with corresponding increase and decrease in the transcript abundance of <jats:italic>laccase<\/jats:italic> confirm the involvement of <jats:italic>laccase<\/jats:italic> in lignin biosynthesis. Negligible transcript in phloem compared to the xylem tissue localized its expression in xylem tissue. This demonstrates the involvement of <jats:italic>P. somniferum laccase<\/jats:italic> in lignin biosynthesis of xylem, providing strength to the peduncle\/stem and preventing lodging.<\/jats:p>","DOI":"10.1111\/ppl.12086","type":"journal-article","created":{"date-parts":[[2013,8,20]],"date-time":"2013-08-20T13:34:52Z","timestamp":1377005692000},"page":"436-445","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Latex\u2010less opium poppy: cause for less latex and reduced peduncle strength"],"prefix":"10.1111","volume":"150","author":[{"given":"Nidarshana","family":"Chaturvedi","sequence":"first","affiliation":[{"name":"Biotechnology Division CSIR\u2010Central Institute of Medicinal and Aromatic Plants P.O. CIMAP Lucknow 226015 UP India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharad K.","family":"Singh","sequence":"additional","affiliation":[{"name":"Plant Breeding and Genetics CSIR\u2010Central Institute of Medicinal and Aromatic Plants P.O. CIMAP Lucknow 226015 UP India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashutosh K.","family":"Shukla","sequence":"additional","affiliation":[{"name":"Biotechnology Division CSIR\u2010Central Institute of Medicinal and Aromatic Plants P.O. CIMAP Lucknow 226015 UP India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raj K.","family":"Lal","sequence":"additional","affiliation":[{"name":"Plant Breeding and Genetics CSIR\u2010Central Institute of Medicinal and Aromatic Plants P.O. 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