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The functions and underlying mechanisms of<jats:styled-content style=\"fixed-case\">WRKY<\/jats:styled-content>proteins have been investigated primarily in model plants such as<jats:italic>Arabidopsis<\/jats:italic>and rice. The roles of these transcription factors in non\u2010model plants, including pepper and other Solanaceae, are poorly understood. Here, we characterize the expression and function of a subgroup<jats:styled-content style=\"fixed-case\">IIe WRKY<\/jats:styled-content>protein from pepper (<jats:italic>Capsicum annuum<\/jats:italic>), denoted as<jats:styled-content style=\"fixed-case\">CaWRKY27<\/jats:styled-content>. The protein localized to nuclei and activated the transcription of a reporter<jats:italic><jats:styled-content style=\"fixed-case\">GUS<\/jats:styled-content><\/jats:italic>gene construct driven by the<jats:italic><jats:styled-content style=\"fixed-case\">35S<\/jats:styled-content><\/jats:italic>promoter that contained two copies of the W\u2010box in its proximal upstream region. Inoculation of pepper cultivars with<jats:italic>Ralstonia solanacearum<\/jats:italic>induced the expression of<jats:italic><jats:styled-content style=\"fixed-case\">CaWRKY27<\/jats:styled-content><\/jats:italic>transcript in 76a, a bacterial wilt\u2010resistant pepper cultivar, whereas it downregulated the expression of<jats:italic><jats:styled-content style=\"fixed-case\">CaWRKY27<\/jats:styled-content><\/jats:italic>transcript in Gui\u20101\u20103, a bacterial wilt\u2010susceptible pepper cultivar.<jats:italic><jats:styled-content style=\"fixed-case\">CaWRKY27<\/jats:styled-content><\/jats:italic>transcript levels were also increased by treatments with salicylic acid (<jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>), methyl jasmonate (<jats:styled-content style=\"fixed-case\">MeJA<\/jats:styled-content>) and ethephon (<jats:styled-content style=\"fixed-case\">ETH<\/jats:styled-content>). Transgenic tobacco plants overexpressing<jats:italic><jats:styled-content style=\"fixed-case\">CaWRKY27<\/jats:styled-content><\/jats:italic>exhibited resistance to<jats:italic>R. solanacearum<\/jats:italic>infection compared to that of wild\u2010type plants. This resistance was coupled with increased transcript levels in a number of marker genes, including hypersensitive response genes, and<jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>\u2010,<jats:styled-content style=\"fixed-case\">JA<\/jats:styled-content>\u2010 and<jats:styled-content style=\"fixed-case\">ET<\/jats:styled-content>\u2010associated genes. By contrast, virus\u2010induced gene silencing (<jats:styled-content style=\"fixed-case\">VIGS<\/jats:styled-content>) of<jats:italic><jats:styled-content style=\"fixed-case\">CaWRKY27<\/jats:styled-content><\/jats:italic>increased the susceptibility of pepper plants to<jats:italic>R. solanacearum<\/jats:italic>infection. These results suggest that<jats:italic><jats:styled-content style=\"fixed-case\">CaWRKY27<\/jats:styled-content><\/jats:italic>acts as a positive regulator in tobacco resistance responses to<jats:italic>R. solanacearum<\/jats:italic>infection through modulation of<jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>\u2010,<jats:styled-content style=\"fixed-case\">JA<\/jats:styled-content>\u2010 and<jats:styled-content style=\"fixed-case\">ET<\/jats:styled-content>\u2010mediated signaling pathways.<\/jats:p>","DOI":"10.1111\/ppl.12093","type":"journal-article","created":{"date-parts":[[2013,8,28]],"date-time":"2013-08-28T16:17:38Z","timestamp":1377706658000},"page":"397-411","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":158,"title":["Overexpression of<i><scp>CaWRKY27<\/scp><\/i>, a subgroup<scp>IIe WRKY<\/scp>transcription factor of<i>Capsicum annuum<\/i>, positively regulates tobacco resistance to<i>Ralstonia solanacearum<\/i>infection"],"prefix":"10.1111","volume":"150","author":[{"given":"Fengfeng","family":"Dang","sequence":"first","affiliation":[{"name":"National Education Minster Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization Fujian Agriculture and Forestry University Fuzhou Fujian 350002 China"},{"name":"College of Life Science Fujian Agriculture and Forestry University Fuzhou Fujian 350002 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuna","family":"Wang","sequence":"additional","affiliation":[{"name":"National Education Minster Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization Fujian Agriculture and Forestry University Fuzhou Fujian 350002 China"},{"name":"Department of Life Science Luoyang Normal University Luoyang Henan 471022 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianju","family":"She","sequence":"additional","affiliation":[{"name":"National Education Minster Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization Fujian Agriculture and Forestry University Fuzhou Fujian 350002 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufen","family":"Lei","sequence":"additional","affiliation":[{"name":"National Education Minster Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization Fujian Agriculture and Forestry University Fuzhou Fujian 350002 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqin","family":"Liu","sequence":"additional","affiliation":[{"name":"National Education Minster Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization Fujian Agriculture and Forestry University Fuzhou Fujian 350002 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Eulgem","sequence":"additional","affiliation":[{"name":"Center for Plant Cell Biology, Institute for Integrative Genome Biology, Department of Botany and Plant 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