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Profiling the dynamics of metabolic changes during ripening and CA storage is vital for understanding the governing molecular mechanism. In this study, the dynamics of the primary metabolism of \u2018Jonagold\u2019 apples during ripening in regular air (<jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content>) storage and initiation of<jats:styled-content style=\"fixed-case\">CA<\/jats:styled-content>storage was profiled. 1\u2010Methylcyclopropene (1\u2010<jats:styled-content style=\"fixed-case\">MCP<\/jats:styled-content>) was exploited to block ethylene receptors and to get insight into ethylene mediated metabolic changes during ripening of the fruit and in response to hypoxic stress. Metabolic changes were quantified in glycolysis, the tricarboxylic acid (<jats:styled-content style=\"fixed-case\">TCA<\/jats:styled-content>) cycle, the Yang cycle and synthesis of the main amino acids branching from these metabolic pathways. Partial least square discriminant analysis of the metabolic profiles of 1\u2010<jats:styled-content style=\"fixed-case\">MCP<\/jats:styled-content>treated and control apples revealed a metabolic divergence in ethylene, organic acid, sugar and amino acid metabolism. During<jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content>storage at 18\u00b0C, most amino acids were higher in 1\u2010<jats:styled-content style=\"fixed-case\">MCP<\/jats:styled-content>treated apples, whereas 1\u2010aminocyclopropane\u20101\u2010carboxylic acid (<jats:styled-content style=\"fixed-case\">ACC<\/jats:styled-content>) was higher in the control apples. The initial response of the fruit to<jats:styled-content style=\"fixed-case\">CA<\/jats:styled-content>initiation was accompanied by an increase of alanine, succinate and glutamate, but a decline in aspartate. Furthermore, alanine and succinate accumulated to higher levels in control apples than 1\u2010<jats:styled-content style=\"fixed-case\">MCP<\/jats:styled-content>treated apples. The observed metabolic changes in these interlinked metabolites may indicate a coordinated adaptive strategy to maximize energy production.<\/jats:p>","DOI":"10.1111\/ppl.12351","type":"journal-article","created":{"date-parts":[[2015,5,29]],"date-time":"2015-05-29T17:34:37Z","timestamp":1432920877000},"page":"232-247","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Metabolic profiling reveals ethylene mediated metabolic changes and a coordinated adaptive mechanism of \u2018Jonagold\u2019 apple to low oxygen stress"],"prefix":"10.1111","volume":"155","author":[{"given":"Elias A.","family":"Bekele","sequence":"first","affiliation":[{"name":"Department of Biosystems (BIOSYST), Division of Mechatronics, Biostatistics and Sensors (MeBioS) KU Leuven Willem de Croylaan 42, bus 2428 3001 Leuven Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wasiye 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