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In angiosperms (Magnoliophyta), <jats:styled-content style=\"fixed-case\">ABA<\/jats:styled-content>\u2010induced gene expression is mediated by promoter elements such as the G\u2010box\u2010like <jats:styled-content style=\"fixed-case\">ACGT<\/jats:styled-content>\u2010core motifs recognized by <jats:styled-content style=\"fixed-case\">bZIP<\/jats:styled-content> transcription factors. In contrast, the mode of regulation by <jats:styled-content style=\"fixed-case\">ABA<\/jats:styled-content> of gene expression in liverworts (Marchantiophyta), representing one of the earliest diverging land plant groups, has not been elucidated. In this study, we used promoters of the liverwort <jats:italic>Marchantia polymorpha<\/jats:italic> dehydrin and the wheat <jats:italic>Em<\/jats:italic> genes fused to the \u03b2\u2010glucuronidase (<jats:italic><jats:styled-content style=\"fixed-case\">GUS<\/jats:styled-content><\/jats:italic>) reporter gene to investigate <jats:styled-content style=\"fixed-case\">ABA<\/jats:styled-content>\u2010induced gene expression in liverworts. Transient assays of cultured cells of Marchantia indicated that <jats:styled-content style=\"fixed-case\">ACGT<\/jats:styled-content>\u2010core motifs proximal to the transcription initiation site play a role in the <jats:styled-content style=\"fixed-case\">ABA<\/jats:styled-content>\u2010induced gene expression. The <jats:styled-content style=\"fixed-case\">RY<\/jats:styled-content> sequence recognized by <jats:styled-content style=\"fixed-case\">B3<\/jats:styled-content> transcriptional regulators was also shown to be responsible for the <jats:styled-content style=\"fixed-case\">ABA<\/jats:styled-content>\u2010induced gene expression. In transgenic Marchantia plants, <jats:styled-content style=\"fixed-case\">ABA<\/jats:styled-content> treatment elicited an increase in <jats:styled-content style=\"fixed-case\">GUS<\/jats:styled-content> expression in young gemmalings, which was abolished by simultaneous disruption of the <jats:styled-content style=\"fixed-case\">ACGT<\/jats:styled-content>\u2010core and <jats:styled-content style=\"fixed-case\">RY<\/jats:styled-content> elements. <jats:styled-content style=\"fixed-case\">ABA<\/jats:styled-content>\u2010induced <jats:styled-content style=\"fixed-case\">GUS<\/jats:styled-content> expression was less obvious in mature thalli than in young gemmalings, associated with reductions in sensitivity to exogenous <jats:styled-content style=\"fixed-case\">ABA<\/jats:styled-content> during gametophyte growth. In contrast, lunularic acid, which had been suggested to function as an <jats:styled-content style=\"fixed-case\">ABA<\/jats:styled-content>\u2010like substance, had no effect on <jats:styled-content style=\"fixed-case\">GUS<\/jats:styled-content> expression. The results demonstrate the presence of <jats:styled-content style=\"fixed-case\">ABA<\/jats:styled-content>\u2010specific response mechanisms mediated by conserved <jats:italic>cis<\/jats:italic>\u2010regulatory elements in liverworts, implying that the mechanisms had been acquired in the common ancestors of embryophytes.<\/jats:p>","DOI":"10.1111\/ppl.12385","type":"journal-article","created":{"date-parts":[[2015,9,10]],"date-time":"2015-09-10T08:15:14Z","timestamp":1441872914000},"page":"407-420","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Abscisic acid\u2010induced gene expression in the liverwort <i>Marchantia polymorpha<\/i> is mediated by evolutionarily conserved promoter elements"],"prefix":"10.1111","volume":"156","author":[{"given":"Totan K.","family":"Ghosh","sequence":"first","affiliation":[{"name":"Graduate School of Science and Engineering Saitama University  Saitama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Midori","family":"Kaneko","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Engineering Saitama University  Saitama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khaleda","family":"Akter","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Engineering Saitama University  Saitama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuhei","family":"Murai","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Engineering Saitama University  Saitama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Komatsu","sequence":"additional","affiliation":[{"name":"Department of Bioproduction Technology Junior College of Tokyo University of Agriculture  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimitsune","family":"Ishizaki","sequence":"additional","affiliation":[{"name":"Graduate School of Science Kobe University  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuyuki T.","family":"Yamato","sequence":"additional","affiliation":[{"name":"Faculty of Biology\u2010Oriented Science and Engineering Kinki University  Higashiosaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takayuki","family":"Kohchi","sequence":"additional","affiliation":[{"name":"Graduate School of Biostudies Kyoto University  Kyoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisuke","family":"Takezawa","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Engineering Saitama University  Saitama Japan"},{"name":"Institute for Environmental Science and Technology Saitama University  Saitama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,10,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jplph.2014.05.004"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.007096"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pp.45.060194.000553"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.1996.tb00546.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002944"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/9783527628964.ch2"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1979.tb13248.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2007.02187.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.12.4.599"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.105.035659"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10265-011-0412-3"},{"key":"e_1_2_8_13_1","first-page":"171","volume-title":"Bryophytes: Their Chemistry and Chemical Taxonomy. 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