{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:19:36Z","timestamp":1787015976026,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,11,16]],"date-time":"2015-11-16T00:00:00Z","timestamp":1447632000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Ribosome\u2010inactivating proteins (\n                    <jats:styled-content style=\"fixed-case\">RIPs<\/jats:styled-content>\n                    ) are a class of plant defense proteins with N\u2010glycosidase activity (\n                    <jats:styled-content style=\"fixed-case\">EC<\/jats:styled-content>\n                    3.2.2.22). Pokeweed antiviral protein (\n                    <jats:styled-content style=\"fixed-case\">PAP<\/jats:styled-content>\n                    ) is a Type I\n                    <jats:styled-content style=\"fixed-case\">RIP<\/jats:styled-content>\n                    isolated from the pokeweed plant,\n                    <jats:italic>Phytolacca americana<\/jats:italic>\n                    , thought to confer broad\u2010spectrum virus resistance in this plant. Through a combination of standard molecular techniques and\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    sequencing analysis, we report here that a small\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    binds and cleaves the open reading frame of\n                    <jats:styled-content style=\"fixed-case\">PAP mRNA<\/jats:styled-content>\n                    . Additionally,\n                    <jats:styled-content style=\"fixed-case\">sRNA<\/jats:styled-content>\n                    targeting of\n                    <jats:styled-content style=\"fixed-case\">PAP<\/jats:styled-content>\n                    is dependent on jasmonic acid (JA), a plant hormone important for defense against pathogen infection and herbivory. Levels of small\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    increased with JA treatment, as did levels of\n                    <jats:styled-content style=\"fixed-case\">PAP mRNA<\/jats:styled-content>\n                    and protein, suggesting that the small\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    functions to moderate the expression of\n                    <jats:styled-content style=\"fixed-case\">PAP<\/jats:styled-content>\n                    in response to this hormone. The association between JA and\n                    <jats:styled-content style=\"fixed-case\">PAP<\/jats:styled-content>\n                    expression, mediated by\n                    <jats:styled-content style=\"fixed-case\">sRNA299<\/jats:styled-content>\n                    , situates\n                    <jats:styled-content style=\"fixed-case\">PAP<\/jats:styled-content>\n                    within a signaling pathway initiated by biotic stress. The consensus sequence of\n                    <jats:styled-content style=\"fixed-case\">sRNA299<\/jats:styled-content>\n                    was obtained through bioinformatic analysis of pokeweed small\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    sequencing. To our knowledge, this is the first account of a\n                    <jats:styled-content style=\"fixed-case\">sRNA<\/jats:styled-content>\n                    targeting a\n                    <jats:styled-content style=\"fixed-case\">RIP<\/jats:styled-content>\n                    gene.\n                  <\/jats:p>","DOI":"10.1111\/ppl.12393","type":"journal-article","created":{"date-parts":[[2015,10,9]],"date-time":"2015-10-09T06:53:06Z","timestamp":1444373586000},"page":"241-251","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A small\n                    <scp>RNA<\/scp>\n                    targets pokeweed antiviral protein transcript"],"prefix":"10.1111","volume":"156","author":[{"given":"Alexander","family":"Klenov","sequence":"first","affiliation":[{"name":"Department of Biology York University  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kira C. M.","family":"Neller","sequence":"additional","affiliation":[{"name":"Department of Biology York University  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydia A.","family":"Burns","sequence":"additional","affiliation":[{"name":"Department of Biology York University  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Krivdova","sequence":"additional","affiliation":[{"name":"Department of Biology York University  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katalin A.","family":"Hudak","sequence":"additional","affiliation":[{"name":"Department of Biology York University  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,11,16]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-arplant-050213-035728"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.12581"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-014-0204-y"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12817"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)00645-6"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(88)90733-4"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1417"},{"key":"e_1_2_8_9_1","doi-asserted-by":"crossref","first-page":"3251","DOI":"10.1016\/S0021-9258(19)85688-0","article-title":"Inhibition of elongation factor 2\u2010dependent translocation by the pokeweed antiviral protein and ricin","volume":"255","author":"Gessner SL","year":"1980","journal-title":"J Biol Chem"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/379777b0"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2229-13-119"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a003092"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/ern030"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(75)90195-2"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(80)90372-0"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn896"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00027159"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12250-011-3223-8"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2008.12.020"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.2008.01129.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2008.03690.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2011.05.001"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02366"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00233148"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2010.07.020"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2009-10-3-r25"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.0030233"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.15.7089"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-012-1734-7"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1415-47572013005000005"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4722"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.106.045617"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-008-0796-z"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-760"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02703672"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1515\/znc-2010-5-607"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.0922"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006444322626"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746-4811-3-12"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-003-1014-7"}],"container-title":["Physiologia Plantarum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fppl.12393","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ppl.12393","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T13:42:18Z","timestamp":1695994938000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ppl.12393"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,11,16]]},"references-count":39,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2016,3]]}},"alternative-id":["10.1111\/ppl.12393"],"URL":"https:\/\/doi.org\/10.1111\/ppl.12393","archive":["Portico"],"relation":{},"ISSN":["0031-9317","1399-3054"],"issn-type":[{"value":"0031-9317","type":"print"},{"value":"1399-3054","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,11,16]]},"assertion":[{"value":"2015-07-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-09-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-11-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}