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Cytokinin plays an active role in cell growth division and differentiation. The <jats:italic>R<\/jats:italic>elated to <jats:italic>A<\/jats:italic>BI3\/<jats:italic>V<\/jats:italic>P1 (RAV) family comprises transcription factors in plants and all contain both AP2\u2010 and B3\u2010like domains. In this study, <jats:italic>GmRAV1<\/jats:italic> (<jats:styled-content style=\"fixed-case\"><jats:italic>Glycine max<\/jats:italic><\/jats:styled-content>), which belongs to the AP2\/ERF transcription factor family, was isolated and functionally characterized. Subcellular localization showed that GmRAV1 was localized to the nucleus and quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR) analysis indicated that <jats:italic>GmRAV1<\/jats:italic> was induced by cytokinin. Furthermore, compared with wild\u2010type plants, plants overexpressing <jats:italic>GmRAV1<\/jats:italic> showed dwarfism and late maturity. In contrast, the mutant of <jats:italic>TEMPRANILLO<\/jats:italic> (<jats:italic>tem1<\/jats:italic>) and <jats:italic>GmRAV\u2010i<\/jats:italic> plants had an opposite phenotype. More interestingly, a root and shoot regeneration experiment indicated that <jats:italic>GmRAV1<\/jats:italic> is one of the most important positive regulators of the cytokinin signaling pathway, which is involved in promoting root and shoot regeneration. In addition, RNA\u2010seq and qRT\u2010PCR results indicated that <jats:italic>GmRAV1<\/jats:italic> is related to the key factors involved in the cytokinin signaling pathway, namely, cytokinin oxidase (<jats:italic>GmCKX6<\/jats:italic> and <jats:italic>GmCKX7<\/jats:italic>), purine permease (<jats:italic>GmPUP1<\/jats:italic>), cell cyclin\u2010related genes (<jats:italic>GmCycA2;4<\/jats:italic>, <jats:italic>GmCycD3<\/jats:italic> and <jats:italic>GmCYC1<\/jats:italic>), <jats:italic>c<\/jats:italic>yclin\u2010<jats:italic>d<\/jats:italic>ependent <jats:italic>k<\/jats:italic>inase (<jats:italic>GmCDKB2<\/jats:italic>), <jats:italic>c<\/jats:italic>ell <jats:italic>d<\/jats:italic>ivision <jats:italic>c<\/jats:italic>ycle (<jats:italic>GmCDC20<\/jats:italic>), E2F transcription factors (<jats:italic>GmE2FE<\/jats:italic>) and <jats:italic>a<\/jats:italic>uthentic <jats:italic>r<\/jats:italic>esponse <jats:italic>r<\/jats:italic>egulator (<jats:italic>GmARR9<\/jats:italic>). In conclusion, <jats:italic>GmRAV1<\/jats:italic>, one of the most important positive regulators involved in promoting root and shoot regeneration, was induced by cytokinin and is related to the key factors of the cytokinin signaling pathways.<\/jats:p>","DOI":"10.1111\/ppl.12788","type":"journal-article","created":{"date-parts":[[2018,6,20]],"date-time":"2018-06-20T10:29:42Z","timestamp":1529490582000},"page":"814-829","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["<i>GmRAV1<\/i> regulates regeneration of roots and adventitious buds by the cytokinin signaling pathway in Arabidopsis and soybean"],"prefix":"10.1111","volume":"165","author":[{"given":"Kexin","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory of Soybean Biology in the Chinese Education Ministry, Soybean Research Institute Northeast Agricultural University  Harbin 150030 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