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Under cadmium (Cd) stress conditions, V\u2010ATPase activity is inhibited. In the present work, hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) and hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>) cross\u2010talk was analyzed in cucumber (<jats:styled-content style=\"fixed-case\"><jats:italic>Cucumis sativus<\/jats:italic><\/jats:styled-content> L.) seedlings exposed to Cd to explain the role of both signaling molecules in the control of V\u2010ATPase. V\u2010ATPase activity and gene expression as well as H<jats:sub>2<\/jats:sub>S and H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> content and endogenous production were determined in roots of plants treated with 100\u00a0\u03bcM CdCl<jats:sub>2<\/jats:sub> and different inhibitors or scavengers. It was found that H<jats:sub>2<\/jats:sub>S donor improved photosynthetic parameters in Cd\u2010stressed cucumber seedlings. Cd\u2010induced stimulation of H<jats:sub>2<\/jats:sub>S level was correlated with the increased activities of the H<jats:sub>2<\/jats:sub>S\u2010generating desulfhydrases. Increased H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> and lowered H<jats:sub>2<\/jats:sub>S contents in roots were able to reduce V\u2010ATPase activities similar to Cd. H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> and H<jats:sub>2<\/jats:sub>S\u2010induced modulations in V\u2010ATPase activities were not closely related to the transcript level of encoding genes, suggesting posttranslational modifications of enzyme protein. On the other hand, exogenous H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> raised H<jats:sub>2<\/jats:sub>S content in root tissues independently from the desulfhydrase activity. Although treatment of control plants with H<jats:sub>2<\/jats:sub>S significantly stimulated NADPH oxidase activity and gene expression, H<jats:sub>2<\/jats:sub>S did not affect H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> accumulation in roots exposed to Cd. The results suggest the existence of two pathways of H<jats:sub>2<\/jats:sub>S generation in Cd\u2010stressed cucumber roots. One involves desulfhydrase activity, as was previously demonstrated in different plant species. The other, the desulfhydrase\u2010independent pathway induced by H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\/NADPH oxidase, may protect V\u2010ATPase from inhibition by Cd.<\/jats:p>","DOI":"10.1111\/ppl.12819","type":"journal-article","created":{"date-parts":[[2018,8,18]],"date-time":"2018-08-18T05:29:20Z","timestamp":1534570160000},"page":"688-704","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["Interaction between the signaling molecules hydrogen sulfide and hydrogen peroxide and their role in vacuolar H<sup>+<\/sup>\u2010ATPase regulation in cadmium\u2010stressed cucumber roots"],"prefix":"10.1111","volume":"166","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0421-8018","authenticated-orcid":false,"given":"Katarzyna","family":"Kaba\u0142a","sequence":"first","affiliation":[{"name":"Department of Plant Molecular Physiology Institute of Experimental Biology, University of Wroc\u0142aw  50\u2010328, 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