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Fruit ripening is caused by multiple biochemical pathways in which, reactive oxygen and nitrogen species (ROS\/RNS) metabolism is involved. Previous studies have demonstrated the differential modulation of nitric oxide (NO) and hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) content during sweet pepper (<jats:italic>Capsicum annuum<\/jats:italic> L.) fruit ripening, both of which regulate NADP\u2010isocitrate dehydrogenase activity. To gain a deeper understanding of the potential functions of other NADPH\u2010generating components, we analyzed glucose\u20106\u2010phosphate dehydrogenase (G6PDH) and 6\u2010phosphogluconate dehydrogenase (6PGDH), which are involved in the oxidative phase of the pentose phosphate pathway (OxPPP) and NADP\u2010malic enzyme (NADP\u2010ME). During fruit ripening, G6PDH activity diminished by 38%, while 6PGDH and NADP\u2010ME activity increased 1.5\u2010 and 2.6\u2010fold, respectively. To better understand the potential regulation of these NADP\u2010dehydrogenases by H<jats:sub>2<\/jats:sub>S, we obtained a 50\u201375% ammonium\u2010sulfate\u2010enriched protein fraction containing these proteins. With the aid of in vitro assays, in the presence of H<jats:sub>2<\/jats:sub>S, we observed that, while NADP\u2010ME activity was inhibited by up to 29\u201332% using 2 and 5\u00a0mM Na<jats:sub>2<\/jats:sub>S as H<jats:sub>2<\/jats:sub>S donor, G6PDH and 6PGDH activities were unaffected. On the other hand, NO donors, <jats:italic>S<\/jats:italic>\u2010nitrosocyteine (CysNO) and DETA NONOate also inhibited NADP\u2010ME activity by 35%. These findings suggest that both NADP\u2010ME and 6PGDH play an important role in maintaining the supply of NADPH during pepper fruit ripening and that H<jats:sub>2<\/jats:sub>S and NO partially modulate the NADPH\u2010generating system.<\/jats:p>","DOI":"10.1111\/ppl.13000","type":"journal-article","created":{"date-parts":[[2019,6,1]],"date-time":"2019-06-01T11:09:13Z","timestamp":1559387353000},"page":"278-288","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":71,"title":["Inhibition of NADP\u2010malic enzyme activity by H<sub>2<\/sub>S and NO in sweet pepper (<i>Capsicum annuum<\/i> L.) fruits"],"prefix":"10.1111","volume":"168","author":[{"given":"Mar\u00eda A.","family":"Mu\u00f1oz\u2010Vargas","sequence":"first","affiliation":[{"name":"Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estaci\u00f3n Experimental del 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