{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T10:51:33Z","timestamp":1783421493937,"version":"3.54.6"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,3,14]],"date-time":"2020-03-14T00:00:00Z","timestamp":1584144000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["752430"],"award-info":[{"award-number":["752430"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:p>\n                    Stable genetic transformation of plants is a low\u2010efficiency process, and identification of positive transformants usually relies on screening for expression of a co\u2010transformed marker gene. Often this involves germinating seeds on solid media containing a selection reagent. Germination on solid media requires surface sterilization of seeds and careful aseptic technique to prevent microbial contamination, but surface sterilization techniques are time consuming and can cause seed mortality if not performed carefully. We developed an antimicrobial cocktail that can be added to solid media to inhibit bacterial and fungal growth without impairing germination, allowing us to bypass the surface sterilization step. Adding a combination of terbinafine (1 \u03bcM) and timentin (200\u2009mg\u2009l\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) to Murashige and Skoog agar delayed the onset of observable microbial growth and did not affect germination of non\u2010sterile seeds from 10 different wild\u2010type and mutant\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    <\/jats:styled-content>\n                    accessions. We named this antimicrobial solid medium \u201cMSTT agar\u201d. Seedlings sown in non\u2010sterile conditions could be maintained on MSTT agar for up to a week without observable contamination. This medium was compatible with rapid screening methods for hygromycin B, phosphinothricin (BASTA) and nourseothricin resistance genes, meaning that positive transformants can be identified from non\u2010sterile seeds in as little as 4\u2009days after stratification, and transferred to soil before the onset of visible microbial contamination. By using MSTT agar we were able to select genetic transformants on solid media without seed surface sterilization, eliminating a tedious and time\u2010consuming step.\n                  <\/jats:p>","DOI":"10.1111\/ppl.13079","type":"journal-article","created":{"date-parts":[[2020,2,25]],"date-time":"2020-02-25T11:04:40Z","timestamp":1582628680000},"page":"586-599","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Antimicrobial solid media for screening non\u2010sterile\n                    <scp>\n                      <i>Arabidopsis thaliana<\/i>\n                    <\/scp>\n                    seeds"],"prefix":"10.1111","volume":"169","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4130-6252","authenticated-orcid":false,"given":"James B.Y.H.","family":"Behrendorff","sequence":"first","affiliation":[{"name":"Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences University of Copenhagen  Frederiksberg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0934-0434","authenticated-orcid":false,"given":"Guillem","family":"Borr\u00e0s\u2010Gas","sequence":"additional","affiliation":[{"name":"Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences University of Copenhagen  Frederiksberg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9174-9548","authenticated-orcid":false,"given":"Mathias","family":"Pribil","sequence":"additional","affiliation":[{"name":"Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences University of Copenhagen  Frederiksberg 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