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The role of exogenous H<jats:sub>2<\/jats:sub>S in regulating plant responses under drought has been reported in a few plant species including spinach, wheat, Arabidopsis, soybean, and citrus plants. However, no report is available on the outcome of exogenous H<jats:sub>2<\/jats:sub>S on drought response in safflower plants. Therefore, the present study was planned to get insight into H<jats:sub>2<\/jats:sub>S\u2010mediated regulation of growth, secondary metabolism, oxidative defense, and uptake of minerals in two safflower cultivars (Safflower\u201016427 and Safflower\u201016493). Plants were exposed to two NaHS (0.5 and 1.0\u2009mM) and two drought levels (70 and 50% field capacity [FC]). We found a notable depression in growth, yield, chlorophyll, and potassium (K<jats:sup>+<\/jats:sup>) uptake under drought. The decline was more significant in plants facing 50% FC. The oxidative injury in plants was higher under severe drought and led to the decline in chlorophyll, plant biomass, and yield production. Drought induced a noticeable accretion in the accumulation of total soluble sugars, proline, ascorbic acid, anthocyanins, and secondary metabolites that protect plants against oxidative damages caused by drought. The activities of antioxidant enzymes increased substantially in safflower cultivars under drought. Besides, plants pretreated with NaHS (0.5\u2009mM) subsided the oxidative damage by increasing the accumulation of secondary metabolites and strengthening the antioxidant capacity under drought. Further, drought plants suffered significant disturbances in ions homeostasis that was circumvented by exogenous H<jats:sub>2<\/jats:sub>S. The interactive effect of drought and H<jats:sub>2<\/jats:sub>S did not display a significant difference between the cultivars.<\/jats:p>","DOI":"10.1111\/ppl.13267","type":"journal-article","created":{"date-parts":[[2020,11,12]],"date-time":"2020-11-12T04:41:39Z","timestamp":1605156099000},"page":"795-808","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Hydrogen sulfide mediates defense response in safflower by regulating secondary metabolism, oxidative defense, and elemental uptake under drought"],"prefix":"10.1111","volume":"172","author":[{"given":"Shahzada B.","family":"Amir","sequence":"first","affiliation":[{"name":"Department of Botany, Faculty of Life Sciences Government College University Faisalabad  Faisalabad 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