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However, research on the H<jats:sub>2<\/jats:sub>S synthase (HSase) genes is still limited in the model legume plant <jats:italic>Medicago truncatula<\/jats:italic> Gaertn. In the present study, a total of 40 HSase family members were first identified and analyzed in the <jats:italic>M. truncatula<\/jats:italic> genome, and these genes distributed across eight chromosomes and were clustered into five groups (I\u2013V) based on their conserved gene structures and protein motifs. Expression analysis revealed that the MtHSase genes were expressed in all the tested abiotic stresses, albeit with expression level differences. This study also showed that H<jats:sub>2<\/jats:sub>S improves low temperature tolerance of alfalfa seedlings by regulating the antioxidant defense system and enhancing photosynthetic capacity. Thus, the study provides new insights into how the H<jats:sub>2<\/jats:sub>S signal regulates tolerance to low\u2010temperature stress and provides the basis for further gene function and detection.<\/jats:p>","DOI":"10.1111\/ppl.13885","type":"journal-article","created":{"date-parts":[[2023,2,28]],"date-time":"2023-02-28T10:28:35Z","timestamp":1677580115000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Integrated bioinformatic and physiological analyses reveal the pivotal role of hydrogen sulfide in enhancing low\u2010temperature tolerance in alfalfa"],"prefix":"10.1111","volume":"175","author":[{"given":"Shuanghong","family":"Gao","sequence":"first","affiliation":[{"name":"College of Grassland Agriculture Northwest A&amp;F University  Yangling People's Republic of 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