{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T04:36:14Z","timestamp":1768624574841,"version":"3.49.0"},"reference-count":16,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,6,20]],"date-time":"2023-06-20T00:00:00Z","timestamp":1687219200000},"content-version":"vor","delay-in-days":50,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100013058","name":"Jiangsu Provincial Key Research and Development Program","doi-asserted-by":"publisher","award":["BE2021366"],"award-info":[{"award-number":["BE2021366"]}],"id":[{"id":"10.13039\/501100013058","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The female\u2010specifically expressed response regulator (<jats:italic>PdFERR<\/jats:italic>) gene in <jats:italic>Populus deltoides<\/jats:italic>, a sex determination gene (an orthologous gene of <jats:italic>ARR17<\/jats:italic> in <jats:italic>Populus tremula<\/jats:italic>), was found to promote femaleness in heterologous expression lines of <jats:italic>Arabidopsis.<\/jats:italic> None of the genes in the <jats:italic>Arabidopsis<\/jats:italic> genome seem to be orthologous to <jats:italic>PdFERR<\/jats:italic>. Although originating from two evolutionarily distant plants, the dioecious poplar <jats:italic>FERR<\/jats:italic> might promote femaleness in the hermaphroditic <jats:italic>Arabidopsis<\/jats:italic> through an evolutionary consistent regulatory pathway. However, there is no molecular evidence to support this viewpoint. In this study, to identify the shared downstream orthologous gene of <jats:italic>PdFERR<\/jats:italic>, we used yeast two\u2010hybrid assay to screen potential interactors of PdFERR in <jats:italic>Arabidopsis<\/jats:italic>. We identified the ethylene response factor 96 (AtERF96) and confirmed the interaction via in vivo and in vitro assays. The <jats:italic>ERF96<\/jats:italic> orthologous gene in <jats:italic>P<\/jats:italic>. <jats:italic>deltoides<\/jats:italic> was also experimentally confirmed to interact with PdFERR. PdFERR could then promote femaleness in poplar or <jats:italic>Arabidopsis<\/jats:italic> through interactions with ERF96, which provide a new perspective for understanding the <jats:italic>PdFERR<\/jats:italic> gene regulating sex differentiation.<\/jats:p>","DOI":"10.1111\/ppl.13948","type":"journal-article","created":{"date-parts":[[2023,6,9]],"date-time":"2023-06-09T03:10:10Z","timestamp":1686280210000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The sex determination gene of <scp><i>Populus deltoides<\/i><\/scp>, <scp><i>PdFERR<\/i><\/scp>, interacts with <scp><i>ERF96<\/i><\/scp> to promote the development of female flower organs"],"prefix":"10.1111","volume":"175","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7078-2595","authenticated-orcid":false,"given":"Jing","family":"Lu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, Co\u2010Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Tree Genetics and Biotechnology of Educational Department of China, Key Laboratory of Tree Genetics and Silvicultural Sciences of Jiangsu Province Nanjing Forestry University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8647-1158","authenticated-orcid":false,"given":"Yonghua","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute for Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences Nanjing University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6273-6193","authenticated-orcid":false,"given":"Tongming","family":"Yin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, Co\u2010Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Tree Genetics and Biotechnology of Educational Department of China, Key Laboratory of Tree Genetics and Silvicultural Sciences of Jiangsu Province Nanjing Forestry University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,6,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.12583"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1189604"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2017.01841"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.13"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2020.02.024"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2017.01841"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2023.116336"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.14402"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3732\/ajb.92.7.1068"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477-020-0672-9"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10535-017-0734-7"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2015.01064"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-19559-2"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2022.02.002"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.199"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.114.243360"}],"container-title":["Physiologia Plantarum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ppl.13948","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T17:30:49Z","timestamp":1692552649000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ppl.13948"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5]]},"references-count":16,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,5]]}},"alternative-id":["10.1111\/ppl.13948"],"URL":"https:\/\/doi.org\/10.1111\/ppl.13948","archive":["Portico"],"relation":{},"ISSN":["0031-9317","1399-3054"],"issn-type":[{"value":"0031-9317","type":"print"},{"value":"1399-3054","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5]]},"assertion":[{"value":"2023-04-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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