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However, little is known about its regulation or function. In this study, we found that transcription of the tomato (<jats:italic>Solanum lycopersicum<\/jats:italic>) <jats:italic>KED<\/jats:italic> gene, <jats:italic>SlKED<\/jats:italic>, was rapidly and transiently elevated by wounding or ethephon treatment. Compared to the wild\u2010type plants, the CRISPR\/Cas9\u2010mediated <jats:italic>SlKED<\/jats:italic> knockout plants did not exhibit altered expression patterns for genes involved in hormone biosynthesis or stress signaling, suggesting a lack of pleiotropic effect on other stress\u2010responsive genes. Conversely, jasmonic acid did not appear to directly regulate <jats:italic>SlKED<\/jats:italic> expression. Wounded leaves of the KED\u2010lacking plants exhibited higher binding of Evans blue dye than the wild\u2010type, indicating a possible role for KED in healing damaged tissues. The <jats:italic>SlKED<\/jats:italic> knockout plants showed a similar dietary effect as the wild\u2010type on the larval growth of tobacco hornworm. But a higher frequency of larval mandible (mouth) movement was recorded during the first 2\u2009minutes of feeding on the wounded KED\u2010lacking <jats:italic>SlKED<\/jats:italic> knockout plants than on the wounded KED\u2010producing wild\u2010type plants, probably reflecting an initial differential response by the feeding larvae to the <jats:italic>SlKED<\/jats:italic> knockout plants. Our findings suggest that <jats:italic>SlKED<\/jats:italic> may be an ethylene\u2010mediated early responder to mechanical stress in tomato, acting downstream of the wound stress response pathways. Although its possible involvement in response to other biotic and abiotic stresses is still unclear, we propose that <jats:italic>SlKED<\/jats:italic> may play a role in plant's rapid, short\u2010term, early wounding responses, such as in cellular damage healing.<\/jats:p>","DOI":"10.1111\/ppl.13978","type":"journal-article","created":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T03:40:58Z","timestamp":1690342858000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp><i>KED<\/i><\/scp> gene expression in early response to wounding stress in tomato plants"],"prefix":"10.1111","volume":"175","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9261-4242","authenticated-orcid":false,"given":"Xing\u2010Hai","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Biological Sciences Florida Atlantic University  Boca Raton Florida 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