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The influence of AMF spore density on plant development is fundamental information to determining the degree of benefits in this relationship. Therefore, the objective of this study was to investigate the effects of different AMF spore densities on thirty\u2010day\u2010old <jats:italic>H. serratifolius<\/jats:italic> seedlings, focusing on growth and biochemical parameters using a completely randomized experimental design with three different spore densities and control. The spore densities were classified as low, medium, and high, with 1.54, 3.08, and 12.35 spores g<jats:sup>\u22121<\/jats:sup>, respectively. Plant growth analysis, mycorrhizal colonization, nitrogen compound concentration, and carbohydrate analysis were performed. The medium spore density treatment showed the greatest increases in biomass, height, leaf area, and root volume. Furthermore, greater absorption of phosphorus and better dynamics in nitrogen metabolism were observed in mycorrhizal plants compared to the control since the ammonium and nitrate compounds were rapidly incorporated into protein and chlorophyll compounds. The carbohydrate analysis revealed the influence of source\u2010sink dynamics on sugar concentration in different plant parts. These findings support the importance of determining the appropriate spore density for assessing the symbiotic relationship between forest species and AMF.<\/jats:p>","DOI":"10.1111\/ppl.14067","type":"journal-article","created":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T22:03:59Z","timestamp":1700604239000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Influence of arbuscular mycorrhizal fungi density on growth and metabolism of <i>Handroanthus serratifolius<\/i> (Vahl) S.O. Grose seedlings"],"prefix":"10.1111","volume":"175","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8187-8576","authenticated-orcid":false,"given":"Ludyanne da Silva","family":"da Sousa","sequence":"first","affiliation":[{"name":"Laboratory of Plant Physiology and Plant Growth Federal University of Western Par\u00e1  Santar\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiane Santos","family":"Correia","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Physiology and Plant Growth Federal University of Western Par\u00e1  Santar\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank dos Santos","family":"dos Farias","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Physiology and Plant Growth Federal University of Western Par\u00e1  Santar\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Diones Ferreira","family":"Santana","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Physiology and Plant Growth Federal University of Western Par\u00e1  Santar\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T\u00falio Silva","family":"Lara","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Physiology and Plant Growth Federal University of Western Par\u00e1  Santar\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,11,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2022.03.051"},{"key":"e_1_2_9_3_1","first-page":"1","article-title":"La biofertilizaci\u00f3n del cacao (Theobroma cacao L.) en vivero con Azospirillum brasilense Tarrand, Krieg et D\u00f6bereiner y Glomus intraradices Schenk et Smith","volume":"32","author":"Aguirre\u2010Medina J.F.","year":"2007","journal-title":"Interciencia"},{"issue":"3","key":"e_1_2_9_4_1","first-page":"331","article-title":"Tabebuia donnell\u2010smithii Rose growth inoculated with mycorrhizal fungi and Azospirillum brasilense","volume":"48","author":"Aguirre\u2010Medina J. 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