{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:51:18Z","timestamp":1791982278397,"version":"4.4.0"},"reference-count":61,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T00:00:00Z","timestamp":1721174400000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Small secreted peptides (SSPs), serving as signaling molecules for intercellular communication, play significant regulatory roles in plant growth, development, pathogen immunity, and responses to abiotic stress. Despite several SSPs, such as PIP, PSK, and PSY having been identified to participate in plant immunity, the majority of SSPs remain understudied, necessitating the exploration and identification of SSPs regulating plant immunity from vast genomic resources. Here we systematically characterized 756 putative SSPs across the genome of <jats:italic>Nicotiana tabacum<\/jats:italic>. 173 SSPs were further annotated as established SSPs, such as nsLTP, CAPE, and CEP. Furthermore, we detected the expression of 484 putative <jats:italic>SSP<\/jats:italic> genes in five tissues, with 83 <jats:italic>SSPs<\/jats:italic> displaying tissue\u2010specific expression. Transcriptomic analysis of tobacco roots under plant defense hormones revealed that 46 <jats:italic>SSP<\/jats:italic>s exhibited specific responsiveness to salicylic acid (SA), and such response was antagonistically regulated by methyl jasmonate. It's worth noting that among these 46 <jats:italic>SSP<\/jats:italic>s, 16 members belong to nsLTP family, and one of them, <jats:italic>NtLTP25<\/jats:italic>, was discovered to enhance tobacco's resistance against <jats:italic>Phytophthora nicotianae<\/jats:italic>. Overexpression of <jats:italic>NtLTP25<\/jats:italic> in tobacco enhanced the expression of <jats:italic>ICS1<\/jats:italic>, subsequently stimulating the biosynthesis of SA and the expression of <jats:italic>NPR1<\/jats:italic> and pathogenesis\u2010related genes. Concurrently, <jats:italic>NtLTP25<\/jats:italic> overexpression activated genes associated with ROS scavenging, consequently mitigating the accumulation of ROS during the subsequent phases of pathogenesis. These discoveries indicate that these 46 SSPs, especially the 16 <jats:italic>nsLTP<\/jats:italic>s, might have a vital role in governing plant immunity that relies on SA signaling. This offers a valuable source for pinpointing SSPs involved in regulating plant immunity.<\/jats:p>","DOI":"10.1111\/ppl.14436","type":"journal-article","created":{"date-parts":[[2024,7,18]],"date-time":"2024-07-18T01:55:40Z","timestamp":1721267740000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Genome\u2010wide Characterization of Small Secreted Peptides in <i>Nicotiana tabacum<\/i> and Functional Assessment of <scp>NtLTP25<\/scp> in Plant Immunity"],"prefix":"10.1111","volume":"176","author":[{"given":"Han","family":"Li","sequence":"first","affiliation":[{"name":"Upland Flue\u2010cured Tobacco Quality and Ecology Key Laboratory, Guizhou Academy of Tobacco Science  Guiyang P. R. 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Upland Flue\u2010cured Tobacco Quality and Ecology Key Laboratory, Guizhou Academy of Tobacco Science  Guiyang P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyao","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Tobacco Science, Guizhou University  Guiyang P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changying","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering, Chengdu University  Chengdu P.R. 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