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This study delves into the characterization of RLCK VII\u20108 members within cucurbits, particularly in melon, examining both structural features and the phylogenetic relationships of these genes\/proteins. The investigation extends to their potential involvement in disease resistance by employing ectopic overexpression in <jats:italic>Arabidopsis<\/jats:italic>. The promoters of CmRLCK VII\u20108 genes harbor multiple phytohormone\u2010 and stress\u2010responsive <jats:italic>cis<\/jats:italic>\u2010acting elements, with the majority (excluding <jats:italic>CmRLCK39<\/jats:italic>) displaying upregulated expression in response to defense hormones and fungal infection. Subcellular localization studies reveal that CmRLCK VII\u20108 proteins predominantly reside on the plasma membrane, with CmRLCK29 and CmRLCK30 exhibiting additional nuclear distribution. Notably, <jats:italic>Arabidopsis<\/jats:italic> plants overexpressing <jats:italic>CmRLCK30<\/jats:italic> manifest dwarfing and delayed flowering phenotypes. Overexpression of <jats:italic>CmRLCK27<\/jats:italic>, <jats:italic>CmRLCK30<\/jats:italic>, and <jats:italic>CmRLCK34<\/jats:italic> in <jats:italic>Arabidopsis<\/jats:italic> imparts enhanced resistance against <jats:italic>Botrytis cinerea<\/jats:italic> and <jats:italic>Pseudomonas syringae<\/jats:italic> pv. <jats:italic>tomato<\/jats:italic> DC3000, concomitant with the strengthened expression of defense genes and reactive oxygen species accumulation. The CmRLCK VII\u20108 members actively participate in chitin\u2010 and flg22\u2010triggered immune responses. Furthermore, CmRLCK30 interacts with CmMAPKKK1 and CmARFGAP, adding a layer of complexity to the regulatory network. In summary, this functional characterization underscores the regulatory roles of <jats:italic>CmRLCK27<\/jats:italic>, <jats:italic>CmRLCK30<\/jats:italic>, and <jats:italic>CmRLCK34<\/jats:italic> in immune responses by influencing pathogen\u2010induced defense gene expression and ROS accumulation.<\/jats:p>","DOI":"10.1111\/ppl.14456","type":"journal-article","created":{"date-parts":[[2024,7,29]],"date-time":"2024-07-29T08:15:41Z","timestamp":1722240941000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Melon <scp>CmRLCK VII<\/scp>\u20108 kinase genes <scp><i>CmRLCK27<\/i><\/scp>, <scp><i>CmRLCK30<\/i><\/scp> and <scp><i>CmRLCK34<\/i><\/scp> modulate resistance against bacterial and fungal diseases in <i>Arabidopsis<\/i>"],"prefix":"10.1111","volume":"176","author":[{"given":"Ya","family":"Wen","sequence":"first","affiliation":[{"name":"Ministry of Agriculture Key Laboratory of Molecular Biology of Crop 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