{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:02:22Z","timestamp":1791230542888,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T00:00:00Z","timestamp":1726012800000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research, India","doi-asserted-by":"publisher","award":["60 (0115)17\/EMR\u2010II"],"award-info":[{"award-number":["60 (0115)17\/EMR\u2010II"]}],"id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Pathogenesis\u2010related proteins (PR), including osmotins, play a vital role in plant defense, being activated in response to diverse biotic and abiotic stresses. Despite their significance, the mechanistic insights into the role of osmotins in plant defense have not been extensively explored. The present study explores the cloning and characterization of the osmotin gene (\n                    <jats:italic>WsOsm<\/jats:italic>\n                    ) from\n                    <jats:italic>Withania somnifera<\/jats:italic>\n                    , aiming to illuminate its role in plant defense mechanisms. Quantitative real\u2010time PCR analysis revealed significant induction of\n                    <jats:italic>WsOsm<\/jats:italic>\n                    in response to various phytohormones e.g. abscisic acid, salicylic acid, methyl jasmonate, brassinosteroids, and ethrel, as well as biotic and abiotic stresses like heat, cold, salt, and drought. To further elucidate\n                    <jats:italic>WsOsm<\/jats:italic>\n                    's functional role, we overexpressed the gene in\n                    <jats:italic>Nicotiana tabacum<\/jats:italic>\n                    , resulting in heightened resistance against the\n                    <jats:italic>Alternaria solani<\/jats:italic>\n                    pathogen. Additionally, we observed enhancements in shoot length, root length, and root biomass in the transgenic tobacco plants compared to wild plants. Notably, the\n                    <jats:italic>WsOsm\u2010<\/jats:italic>\n                    overexpressing seedlings demonstrated improved salt and drought stress tolerance, particularly at the seedling stage. Confocal histological analysis of H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    and biochemical studies of antioxidant enzyme activities revealed higher levels in the\n                    <jats:italic>WsOsm<\/jats:italic>\n                    overexpressing lines, indicating enhanced antioxidant defense. Furthermore, a pull\u2010down assay and mass spectrometry analysis revealed a potential interaction between WsOsm and defensin, a known antifungal PR protein (WsDF). This suggests a novel role of WsOsm in mediating plant defense responses by interacting with other PR proteins. Overall, these findings pave the way for potential future applications of\n                    <jats:italic>WsOsm<\/jats:italic>\n                    in developing stress\u2010tolerant crops and improving plant defense strategies against pathogens.\n                  <\/jats:p>","DOI":"10.1111\/ppl.14513","type":"journal-article","created":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T00:20:33Z","timestamp":1726100433000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["<i>Withania somnifera<\/i>\n                    osmotin (\n                    <scp>WsOsm<\/scp>\n                    ) confers stress tolerance in tobacco and establishes novel interactions with the defensin protein (\n                    <scp>WsDF<\/scp>\n                    )"],"prefix":"10.1111","volume":"176","author":[{"given":"Varinder","family":"Singh","sequence":"first","affiliation":[{"name":"Department of Biotechnology Guru Nanak Dev University  Amritsar Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vipin","family":"Hallan","sequence":"additional","affiliation":[{"name":"Division of Biotechnology CSIR\u2010Institute of Himalayan Bioresource Technology  Palampur Himachal Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9017-7608","authenticated-orcid":false,"given":"Pratap Kumar","family":"Pati","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Guru Nanak Dev University  Amritsar Punjab 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