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Currently, there is few information on the mechanism by which H<jats:sub>2<\/jats:sub>S may be involved in NO\u2010induced salt tolerance. In this study, cucumber was used to investigate the role of NO and H<jats:sub>2<\/jats:sub>S and their relationship under salt stress. The results showed that the H<jats:sub>2<\/jats:sub>S donor sodium hydrosulfide (NaHS) and the NO donor sodium nitroprusside (SNP) significantly promoted the growth of cucumber seedlings under salt stress. However, hypotaurine (HT, an H<jats:sub>2<\/jats:sub>S scavenger) inhibited the positive effects of SNP under salt stress. Meanwhile, SNP treatment significantly increased the content of endogenous H<jats:sub>2<\/jats:sub>S, the activity of H<jats:sub>2<\/jats:sub>S synthesis\u2010related enzymes LCD, DCD, and CAS, and the expression of H<jats:sub>2<\/jats:sub>S synthesis\u2010related genes (<jats:italic>CsLCD<\/jats:italic> and <jats:italic>CsDCD<\/jats:italic>). In addition, the salt tolerance of cucumber seedlings was largely eliminated after silencing the H<jats:sub>2<\/jats:sub>S synthesis\u2010related genes <jats:italic>CsLCD<\/jats:italic>\/<jats:italic>CsDCD<\/jats:italic>, while overexpression of <jats:italic>CsLCD<\/jats:italic>\/<jats:italic>CsDCD<\/jats:italic> significantly enhanced the salt tolerance. It was further found that SNP enhanced the salt tolerance of <jats:italic>CsLCD<\/jats:italic>\/<jats:italic>CsDCD<\/jats:italic> overexpression lines, but it had no positive effect on the tolerance of <jats:italic>CsLCD<\/jats:italic>\/<jats:italic>CsDCD<\/jats:italic> silencing lines. Therefore, NO might enhance salt tolerance in cucumber seedlings by upregulating the expression of H<jats:sub>2<\/jats:sub>S synthesis\u2010related gene <jats:italic>CsLCD<\/jats:italic>\/<jats:italic>CsDCD<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/ppl.14644","type":"journal-article","created":{"date-parts":[[2024,11,29]],"date-time":"2024-11-29T09:09:22Z","timestamp":1732871362000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp><i>CsLCD<\/i><\/scp> and <scp><i>CsDCD<\/i><\/scp> are involved in nitric oxide\u2010enhanced salt tolerance in cucumber seedlings"],"prefix":"10.1111","volume":"176","author":[{"given":"Ailing","family":"Li","sequence":"first","affiliation":[{"name":"College of Horticulture, Gansu Agricultural University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuelian","family":"Li","sequence":"additional","affiliation":[{"name":"College of Horticulture, Gansu 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