{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T03:06:45Z","timestamp":1789441605714,"version":"build-2803163510"},"reference-count":53,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T00:00:00Z","timestamp":1734480000000},"content-version":"vor","delay-in-days":47,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research, India","doi-asserted-by":"publisher","award":["P\u201081"],"award-info":[{"award-number":["P\u201081"]}],"id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Transgenic <jats:italic>Ocimum sanctum<\/jats:italic> plants were engineered to produce vanillin by overexpressing the VpVAN gene using <jats:italic>Agrobacterium<\/jats:italic>\u2010mediated transformation. Positive transformants developed shoots within 4\u20135\u2009weeks and were transferred to a root induction medium and four independent transformants with no observed adverse effects were kept for anlysis. Quantitative RT\u2010PCR indicated significantly higher VpVAN expression in transgenic lines AG_3 and AG_1, impacting the phenylpropanoid pathway and phenolic compound accumulation. Molecular docking studies indicated ferulic acid's higher binding affinity to vanillin synthase than eugenol. LC\u2013MS\/MS analysis revealed a marked increase in vanillin production in transgenic lines compared to wild type, with AG_3 exhibiting the highest vanillin content (1.98\u2009\u00b1\u20090.0047\u2009mg\/g extract) and AG_1 following (1.49\u2009\u00b1\u20090.0047\u2009mg\/g extract). AG_3 also showed elevated levels of benzoic acid, 4\u2010hydroxy benzyl alcohol, and ferulic acid. This study highlights the potential of metabolic engineering in <jats:italic>O. sanctum<\/jats:italic> for enhanced vanillin production, suggesting pathways for large\u2010scale production of natural vanillin and other valuable compounds in transgenic plants.<\/jats:p>","DOI":"10.1111\/ppl.70005","type":"journal-article","created":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T09:06:37Z","timestamp":1734512797000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Metabolic\u2010Engineering Approach to Enhance Vanillin and Phenolic Compounds in <i>Ocimum Sanctum<\/i> (<scp>CIM<\/scp>\u2010Angana) via <scp>VpVAN<\/scp> Overexpression"],"prefix":"10.1111","volume":"176","author":[{"given":"Zakir","family":"Husain","sequence":"first","affiliation":[{"name":"Plant Biotechnology Division CSIR\u2010Central Institute of Medicinal and Aromatic Plants (CIMAP)  Lucknow Utter Pradesh 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