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They participate in the typical MAPK cascade system and various signal transduction pathways regulating biological processes in plants, during stressful conditions. To date, genome\u2010wide identification of MAP4Ks in cotton has not been reported. In this study, 77\n                    <jats:italic>MAP4K<\/jats:italic>\n                    genes were identified in four\n                    <jats:italic>Gossypium<\/jats:italic>\n                    species. Protein characteristics, gene structures, conserved motifs and gene expression analysis were carried out. Genome\u2010wide or fragment duplication has played an important role in the expansion of the\n                    <jats:italic>GhMAP4K<\/jats:italic>\n                    . Promoter cis\u2010acting elements and expression patterns indicated that GhMAP4Ks are related to plant hormones (ABA, MeJA, GA, IAA, SA) and various stresses (drought, hypothermia and wound). Overexpressing\n                    <jats:italic>GhMAP4K13<\/jats:italic>\n                    in Arabidopsis showed higher stem length in response to drought and salt stress. The wilting degree in virus\u2010induced\n                    <jats:italic>GhMAP4K13<\/jats:italic>\n                    gene silenced plants was substantially greater than wild type plants under drought and salt stress. Transcriptomic analysis showed that most differentially expressed genes were involved in the MAPK signaling pathway, carbon metabolism and porphyrin metabolism. Additionally, transgenic Arabidopsis and VIGS cotton showed that\n                    <jats:italic>GhMAP4K13<\/jats:italic>\n                    was positively responsive to drought and salt stresses. This study will play an important role in understanding the function of the MAP4K gene family in response to abiotic stress in cotton.\n                  <\/jats:p>","DOI":"10.1111\/ppl.70031","type":"journal-article","created":{"date-parts":[[2025,1,2]],"date-time":"2025-01-02T00:13:45Z","timestamp":1735776825000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Identification of the\n                    <scp>\n                      <i>MAP4K<\/i>\n                    <\/scp>\n                    gene family reveals\n                    <scp>\n                      <i>GhMAP4K13<\/i>\n                    <\/scp>\n                    regulates drought and salt stress tolerance in cotton"],"prefix":"10.1111","volume":"177","author":[{"given":"Qing","family":"Zeng","sequence":"first","affiliation":[{"name":"Zhengzhou Research Base, State Key Laboratory of Cotton Bio\u2010breeding and Integrated Utilization, School of Agricultural Sciences Zhengzhou University  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanjia","family":"Peng","sequence":"additional","affiliation":[{"name":"Hunan Institute of Cotton Science  Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Zhengzhou Research Base, State Key Laboratory of Cotton Bio\u2010breeding and Integrated Utilization, School of Agricultural Sciences Zhengzhou University  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Wang","sequence":"additional","affiliation":[{"name":"Zhengzhou Research Base, State Key Laboratory of Cotton Bio\u2010breeding and Integrated Utilization, School of Agricultural Sciences Zhengzhou University  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuke","family":"Lu","sequence":"additional","affiliation":[{"name":"Zhengzhou Research Base, State Key Laboratory of Cotton Bio\u2010breeding and Integrated Utilization, School of Agricultural Sciences Zhengzhou University  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3561-7863","authenticated-orcid":false,"given":"Allah","family":"Bakhsh","sequence":"additional","affiliation":[{"name":"Centre of Excellence in Molecular Biology University of the Punjab  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Li","sequence":"additional","affiliation":[{"name":"Zhengzhou Research Base, State Key Laboratory of Cotton Bio\u2010breeding and Integrated Utilization, School of Agricultural Sciences Zhengzhou University  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bobokhonova Zebinisso","family":"Qaraevna","sequence":"additional","affiliation":[{"name":"Department cotton growing, genetics, breeding and seed Tajik agrarian University named Shirinsho Shotemur Dushanbe  Republic of Tajikistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wuwei","family":"Ye","sequence":"additional","affiliation":[{"name":"Zhengzhou Research Base, State Key Laboratory of Cotton Bio\u2010breeding and Integrated Utilization, School of Agricultural Sciences Zhengzhou University  Zhengzhou China"},{"name":"Western Agricultural Research Center, Chinese Academy of Agricultural Sciences  Changji China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5298-2423","authenticated-orcid":false,"given":"Zujun","family":"Yin","sequence":"additional","affiliation":[{"name":"Zhengzhou Research Base, State Key Laboratory of Cotton Bio\u2010breeding and Integrated Utilization, School of Agricultural Sciences Zhengzhou University  Zhengzhou China"},{"name":"Western Agricultural Research Center, Chinese Academy of Agricultural Sciences  Changji China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,1]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.55.031903.141701"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","unstructured":"ArtimoP JonnalageddaM ArnoldK BaratinD CsardiG deCastroE DuvaudS FlegelV FortierA GasteigerE GrosdidierA HernandezC IoannidisV KuznetsovD LiechtiR MorettiS MostaguirK RedaschiN RossierG XenariosIandStockingerH. 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