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Hydrogen sulfide (H\u2082S), as a signaling molecule, regulates various aspects of plant growth and development. In this study, we examined the role of endogenous H\u2082S in regulating flowering time in\n                    <jats:italic>Arabidopsis<\/jats:italic>\n                    . The\n                    <jats:italic>O<\/jats:italic>\n                    \u2010\n                    <jats:italic>acetylserine thiol lyase a1<\/jats:italic>\n                    (\n                    <jats:italic>oasa1<\/jats:italic>\n                    ) mutant, which has elevated H\u2082S levels due to impaired OASA1 activity that catalyzes the synthesis of Cys from H\n                    <jats:sub>2<\/jats:sub>\n                    S, flowers earlier than wild type (WT). The\n                    <jats:italic>OASA1<\/jats:italic>\n                    overexpression lines (\n                    <jats:italic>OE\u2010OASA1\u2010#33\/#142<\/jats:italic>\n                    ), characterized by reduced H\u2082S levels, show delayed flowering, accompanied by decreased expression of key flowering regulators,\n                    <jats:italic>FLOWERING LOCUS T<\/jats:italic>\n                    (\n                    <jats:italic>FT<\/jats:italic>\n                    ),\n                    <jats:italic>SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1<\/jats:italic>\n                    (\n                    <jats:italic>SOC1<\/jats:italic>\n                    ), and\n                    <jats:italic>AGAMOUS\u2010LIKE24<\/jats:italic>\n                    (\n                    <jats:italic>AGL24<\/jats:italic>\n                    ). Notably, vernalization and short\u2010day (SD) conditions did not affect their flowering patterns. Exogenous H\u2082S and GA\u2083 treatment rescued the delayed flowering phenotype of\n                    <jats:italic>OE\u2010OASA1\u2010#33\/#142<\/jats:italic>\n                    . In\n                    <jats:italic>oasa1<\/jats:italic>\n                    , levels of GA intermediates (GA\n                    <jats:sub>15<\/jats:sub>\n                    and GA\n                    <jats:sub>53<\/jats:sub>\n                    ) were elevated, while their levels were reduced in\n                    <jats:italic>OE\u2010OASA1\u2010#33\/#142<\/jats:italic>\n                    . RT\u2010qPCR analysis showed a significant reduction in the expression of\n                    <jats:italic>GIBBERELLIN 20\u2010OXIDASE 4<\/jats:italic>\n                    (\n                    <jats:italic>GA20ox4<\/jats:italic>\n                    ) in\n                    <jats:italic>OE\u2010OASA1\u2010#33\/#142<\/jats:italic>\n                    compared to WT. Overexpression of\n                    <jats:italic>GA20ox4<\/jats:italic>\n                    (\n                    <jats:italic>OE\u2010GA20ox4\u2010#20\/#30<\/jats:italic>\n                    ) resulted in earlier flowering and partially rescued the delayed flowering phenotype of\n                    <jats:italic>OE\u2010OASA1\u2010#33\/#142<\/jats:italic>\n                    . Additionally, the expression of age pathway\u2010related genes, including miRNA172b and\n                    <jats:italic>SQUAMOSA PROMOTER BINDING PROTEIN\u2010LIKE 3\/4\/5\/9\/15<\/jats:italic>\n                    (\n                    <jats:italic>SPL3\/4\/5\/9\/15<\/jats:italic>\n                    ), was significantly reduced in\n                    <jats:italic>OE\u2010OASA1\u2010#33\/#142<\/jats:italic>\n                    seedlings. These findings suggest that endogenous H\u2082S positively regulates\n                    <jats:italic>GA20ox4<\/jats:italic>\n                    expression, thereby promoting gibberellin synthesis and advancing flowering in Arabidopsis through the GA pathway. Furthermore, the promotion of flowering by H\u2082S appears to be linked to the age pathway.\n                  <\/jats:p>","DOI":"10.1111\/ppl.70084","type":"journal-article","created":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T02:13:56Z","timestamp":1738635236000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Endogenous\n                    <scp>\n                      H\n                      <sub>2<\/sub>\n                      S\n                    <\/scp>\n                    promotes\n                    <i>Arabidopsis<\/i>\n                    flowering through the regulation of\n                    <scp>\n                      <i>GA20ox4<\/i>\n                    <\/scp>\n                    in the gibberellin pathway"],"prefix":"10.1111","volume":"177","author":[{"given":"Lijuan","family":"Xuan","sequence":"first","affiliation":[{"name":"Ministry of Education Key Laboratory of Cell Activities 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