{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T12:03:39Z","timestamp":1792411419248,"version":"4.4.0"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T00:00:00Z","timestamp":1744243200000},"content-version":"vor","delay-in-days":40,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Intracellular communications mediated by cytoplasmic streaming compensate for the slowness of diffusion on large scale distances. In characean internodes, cyclosis serves to smooth concentration gradients related to local structural distinctions, irregular spotted illumination, and patterned profiles of external pH. In dimly lit\n                    <jats:italic>Chara<\/jats:italic>\n                    cells, the fluidic transmission of reducing equivalents from the spot of bright light incidence to a remote analyzed area transiently elevates the actual yield of chlorophyll fluorescence (\n                    <jats:italic>F\u2032<\/jats:italic>\n                    ) under natural acidic zones with little effect on\n                    <jats:italic>F\u2032<\/jats:italic>\n                    under alkaline bands. Here, the natural formation of alkaline zones was imitated by placing the internodal cell part into a solution with a pH of 9.5. Using PAM microfluorometry, we found that chloroplasts located under an alkaline solution retained the perception of reducing equivalents transported with the fluid flow but, in addition, became responsive to another transportable metabolite that promoted strong quenching of both\n                    <jats:italic>F<\/jats:italic>\n                    <jats:italic>\u2032<\/jats:italic>\n                    <jats:sub>m<\/jats:sub>\n                    and\n                    <jats:italic>F\u2032<\/jats:italic>\n                    fluorescence. The superposition of oppositely directed\n                    <jats:italic>F\u2032<\/jats:italic>\n                    responses to distinct cyclosis\u2010transported metabolites resulted in the seeming suppression of microfluidic interactions between distant chloroplasts. The action potential generation did not affect\n                    <jats:italic>F<\/jats:italic>\n                    <jats:italic>\u2032<\/jats:italic>\n                    <jats:sub>m<\/jats:sub>\n                    fluorescence (an indicator of non\u2010photochemical quenching, NPQ) when the cell was bathed at neutral pH but induced strong NPQ in the high pH solution. We propose that the restricted CO\n                    <jats:sub>2<\/jats:sub>\n                    supply at high external pH induces the rearrangement of electron transport to alternative pathways, which elevates the background level of NPQ\u2010promoting metabolite (supposedly H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    ), thus enhancing the chloroplast sensitivity to H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    portions delivered with the fluid flow from the region subjected to intense local light.\n                  <\/jats:p>","DOI":"10.1111\/ppl.70211","type":"journal-article","created":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T07:46:38Z","timestamp":1744271198000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Microfluidic communications in characean internodes at neutral and alkaline external\n                    <scp>pH<\/scp>"],"prefix":"10.1111","volume":"177","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3131-8890","authenticated-orcid":false,"given":"Alexander A.","family":"Bulychev","sequence":"first","affiliation":[{"name":"Department of Biophysics, Faculty of Biology Lomonosov Moscow State University  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia A.","family":"Krupenina","sequence":"additional","affiliation":[{"name":"Department of Biophysics, Faculty of Biology Lomonosov Moscow State University  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2015.00226"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00235795"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-29119-7_11"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01869346"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/32.5.951"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0302-4598(01)00096-4"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppl.13531"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00709-022-01747-0"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2022.05.005"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2024.109078"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2015.01.004"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2017.02.014"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioelechem.2019.05.006"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1134\/S1990747824700193"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00709-018-1255-8"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0006297923100048"},{"issue":"1","key":"e_1_2_9_18_1","first-page":"62","article-title":"Light\u2010induced polar pH changes in leaves of Elodea canadensis: I","volume":"91","author":"Elzenga J. 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