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Hitherto, models of\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sub>CO2<\/jats:sub>\n                    have primarily been based on considerations of respiratory energy demand (particularly ATP) for maintenance and growth purposes. Respiratory ATP synthesis is closely tied to the rate of respiratory O\n                    <jats:sub>2<\/jats:sub>\n                    \u2010uptake (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sub>O2<\/jats:sub>\n                    ), with relative engagement of the alternative oxidase influencing the ATP:O ratio. However, the extent to which respiratory ATP synthesis is coupled to leaf\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sub>CO2<\/jats:sub>\n                    depends on the respiratory quotient (RQ, mol CO\n                    <jats:sub>2<\/jats:sub>\n                    efflux per unit mol O\n                    <jats:sub>2<\/jats:sub>\n                    uptake), with models predicting leaf\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sub>CO2<\/jats:sub>\n                    assuming that the RQ is at unity. Here, we show systematic inter\u2010specific, temporal and temperature\u2010dependent variation in leaf RQ, with values of RQ ranging from 0.51 to 2.2, challenging model assumptions on the RQ. We discuss possible mechanisms underlying the variation in leaf RQ, potential ways forward in terms of new measurement protocols, and perspectives for modelled\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sub>CO2<\/jats:sub>\n                    . Our analyses highlight a range of outstanding research questions that need to be answered before we can mechanistically model leaf\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sub>CO2<\/jats:sub>\n                    at various scales.\n                  <\/jats:p>","DOI":"10.1111\/ppl.70235","type":"journal-article","created":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T00:43:53Z","timestamp":1745282633000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Importance of the leaf respiratory quotient"],"prefix":"10.1111","volume":"177","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9811-9194","authenticated-orcid":false,"given":"Dan","family":"Bruhn","sequence":"first","affiliation":[{"name":"Department of Chemistry and Bioscience Aalborg University  Aalborg 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