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This study explored the potential of this technology in mitigating chromium (Cr) stress in rapeseed using a nano\u2010based system that integrates 100\u2009\u03bcM hydrogen sulphide (H<jats:sub>2<\/jats:sub>S) and 50\u2009\u03bcM manganese nanoparticles (Mn\u2010NPs). This strategy reveals Cr\u2010stress tolerance mechanisms through physiological assessments and transcriptome data analysis. The results demonstrated that Cr stress substantially inhibited rapeseed growth while increasing oxidative damage markers (MDA and ROS levels). Conversely, Mn\u2010NP and H<jats:sub>2<\/jats:sub>S co\u2010treatment significantly mitigated these effects, as shown by: (1) restored growth metrics, (2) improved photosynthetic performance and membrane integrity, (3) optimized Mn\/H<jats:sub>2<\/jats:sub>S homeostasis, and (4) reduced tissue Cr accumulation. The reduction in Cr content was attributed to enhanced Cr\u2010detoxification mechanisms, driven by the upregulation of enzymatic antioxidant activities, like superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase. Transcriptomic profiling revealed marked upregulation of genes involved in core metabolic processes, including photosynthetic pathways, carbon assimilation, secondary metabolite biosynthesis, inositol\/phosphatidylinositol signalling systems, and stress\u2010response networks. Under Cr stress, Mn\u2010NP and H<jats:sub>2<\/jats:sub>S co\u2010treated rapeseed plants displayed enhanced tolerance, highlighting the crucial role of these signalling agents in activating Cr\u2010defence mechanisms. Our findings demonstrate that the integration of nanotechnology and gasotransmitter signalling molecule H<jats:sub>2<\/jats:sub>S presents a novel strategy for enhancing heavy metal tolerance and plant productivity in contaminated soils.<\/jats:p>","DOI":"10.1111\/ppl.70286","type":"journal-article","created":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T04:52:08Z","timestamp":1748407928000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Nano\u2010Manganese and <scp>H<sub>2<\/sub>S<\/scp> Signalling Improve Rapeseed Tolerance to Chromium Stress by Regulating Cellular Metabolism and Downstream Pathways"],"prefix":"10.1111","volume":"177","author":[{"given":"Ahsan","family":"Ayyaz","sequence":"first","affiliation":[{"name":"Institute of Crop Science, Ministry of Agriculture and Rural Affairs Key Laboratory of Spectroscopy Sensing Zhejiang University  Hangzhou 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