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In vascular plants, they are differentiated into stacked and unstacked regions, the grana and stroma lamellae, respectively; they are densely packed with protein complexes performing the light reactions of photosynthesis and generating a proton motive force (pmf). The maintenance of pmf and its utilisation for ATP synthesis requires sealing the TMs at their highly curved regions (CRs). These regions are devoid of chlorophyll\u2010containing proteins but contain the curvature\u2010inducing CURVATURE THYLAKOID1 (CURT1) proteins and are enriched in lipids. Because of the highly curved nature of this region, at the margins of grana and stroma TMs, the molecular organisation of lipid molecules is likely to possess distinct features compared to those in the major TM domains. To clarify this question, we isolated CR fractions from <jats:styled-content style=\"fixed-case\"><jats:italic>Spinacia oleracea<\/jats:italic><\/jats:styled-content> and, using BN\u2010PAGE and western blot analysis, verified that they are enriched in CURT1 proteins and in lipids. The lipid phase behaviour of these fractions was fingerprinted with <jats:sup>31<\/jats:sup>P\u2010NMR spectroscopy, which revealed that the bulk lipid molecules assume a non\u2010bilayer, isotropic lipid phase. This finding underpins the importance of the main, non\u2010bilayer lipid species, monogalactosyldiacylglycerol, of TMs in their self\u2010assembly and functional activity.<\/jats:p>","DOI":"10.1111\/ppl.70289","type":"journal-article","created":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T10:48:41Z","timestamp":1750157321000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Lipid Phase Behaviour of the Curvature Region of\u00a0Thylakoid Membranes of <scp><i>Spinacia oleracea<\/i><\/scp>"],"prefix":"10.1111","volume":"177","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-6822-5664","authenticated-orcid":false,"given":"Kinga","family":"B\u00f6de","sequence":"first","affiliation":[{"name":"Faculty of Science University of Ostrava  Ostrava Czech\u00a0Republic"},{"name":"HUN\u2010REN Biological Research Centre  Szeged 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