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Treatment of Ni (500\u2009\u03bcM NiCl<jats:sub>2<\/jats:sub>) reduced growth parameters and the content of \u03b4\u2010amino levulinic acid (\u03b4\u2010ALA), total chlorophylls, and carotenoids. However, EBL priming and foliar HS application mitigated the decline significantly. Seed priming with EBL and HS supplementation alleviated the Ni\u2010induced decline in nitrate reductase activity, photosynthesis, stomatal conductance, transpiration, and intercellular CO<jats:sub>2<\/jats:sub> concentrations. EBL priming and HS mitigated the decline in nitrogen, potassium, and phosphorus under Ni stress and reduced oxidative stress parameters. A significant increase in osmolytes like glycine betaine and proline was imparted due to EBL priming and foliar HS application in non\u2010stressed and Ni\u2010stressed plants. The activity of \u03b3\u2010glutamyl kinase increased and proline oxidase decreased due to EBL and HS treatments, hence increasing proline accumulation. In addition, the activities of antioxidant enzymes increased significantly due to EBL and HS treatments. The contents of ascorbic acid, reduced glutathione, and total phenols increased more conspicuously due to the combined treatment of EBL and HS in Ni\u2010stressed and non\u2010stressed plants. Total antioxidant activity (measured as DPPH and ABTS scavenging) was higher in EBL\u2010primed and HS\u2010treated plants under non\u2010stressed and stressed conditions. Hence, it is concluded that EBL priming and foliar HS application synergistically regulate tolerance mechanisms to alleviate the damaging effects of Ni.<\/jats:p>","DOI":"10.1111\/ppl.70347","type":"journal-article","created":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T08:10:13Z","timestamp":1750407013000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Combined Effects of Epi\u2010Brassinolide Priming and the Foliar Application of Hydrogen Sulphide in Soybean Under Nickel Stress"],"prefix":"10.1111","volume":"177","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3043-2093","authenticated-orcid":false,"given":"Khalid H.","family":"Alamer","sequence":"first","affiliation":[{"name":"Biological Sciences Department, Faculty of Science and Arts King Abdulaziz University  Rabigh Saudi 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