{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T13:20:43Z","timestamp":1792070443928,"version":"4.4.0"},"reference-count":73,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T00:00:00Z","timestamp":1752451200000},"content-version":"am","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T00:00:00Z","timestamp":1752451200000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P20\u2010GM113126"],"award-info":[{"award-number":["P20\u2010GM113126"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["MCB\u20101933590"],"award-info":[{"award-number":["MCB\u20101933590"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Plant mitochondria are in continuous motion. While providing ATP to other cellular processes, they also constantly consume ATP to move rapidly within the cell. This movement is in part related to taking up, converting and delivering metabolites and energy to and from different parts of the cell. Plant mitochondria have varying amounts of DNA, even within a single cell, from none to the full mitochondrial genome. Because mitochondrial dynamics are altered in an\n                    <jats:italic>Arabidopsis<\/jats:italic>\n                    mutant with disrupted DNA maintenance, we hypothesised that exchanging DNA templates for repair is one of the functions of their movement and interactions. Here, we image mitochondrial DNA by two distinct methods while tracking mitochondrial position to investigate differences in the behaviour of mitochondria with and without DNA in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    <\/jats:styled-content>\n                    . In addition to staining mitochondrial DNA with SYBR Green, we have developed and implemented a fluorescent mitochondrial DNA binding protein that will enable future understanding of mitochondrial dynamics, genome maintenance and replication. We demonstrate that mitochondria without mtDNA have altered physical behaviour and lower immediate connectivity to the rest of the population, further supporting a link between the physical and genetic dynamics of these complex organelles.\n                  <\/jats:p>","DOI":"10.1111\/ppl.70404","type":"journal-article","created":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T08:36:41Z","timestamp":1752482201000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["(\n                    <scp>ID<\/scp>\n                    \u2010\n                    <scp>ICPMB05<\/scp>\n                    ) Running on Empty: Mitochondria Without\n                    <scp>DNA<\/scp>\n                    Exhibit Differential Motility and Connectivity"],"prefix":"10.1111","volume":"177","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1473-8415","authenticated-orcid":false,"given":"Joanna M.","family":"Chustecki","sequence":"first","affiliation":[{"name":"School of Biological Sciences University of Nebraska\u2010Lincoln  Lincoln Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alora Q.","family":"Schneider","sequence":"additional","affiliation":[{"name":"School of Biological Sciences University of Nebraska\u2010Lincoln  Lincoln Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madeleine H.","family":"Faber","sequence":"additional","affiliation":[{"name":"School of Biological Sciences University of Nebraska\u2010Lincoln  Lincoln Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9148-640X","authenticated-orcid":false,"given":"Bara","family":"Altartouri","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Center for Biotechnology University of Nebraska\u2010Lincoln  Lincoln Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1125-3172","authenticated-orcid":false,"given":"Alan C.","family":"Christensen","sequence":"additional","affiliation":[{"name":"School of Biological Sciences University of Nebraska\u2010Lincoln  Lincoln Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg251"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.17.01025"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0401077101"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1586\/14737159.5.2.209"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2012.81"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.109.136853"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.107.113647"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/brv.12373"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2010.03.006"},{"issue":"3","key":"e_1_2_8_11_1","first-page":"385","article-title":"Algorithm AS 91: The Percentage Points of the \u03c72 Distribution","volume":"24","author":"Best D. J.","year":"1975","journal-title":"Journal of the Royal Statistical Society: Series C: Applied Statistics"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.BBAMCR.2006.02.014"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.114.127316"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21010328"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erac250"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2021.04.006"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2023.09.005"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1101\/2023.10.23.563667"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10895\u2010012\u20101059\u20108"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcad098"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/TPJ.14534"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.15.00887"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1017\/qpb.2022.15"},{"key":"e_1_2_8_24_1","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Green M. R.","year":"2012"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2013.09.016"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010arplant\u2010043015\u2010112232"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erw178"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7265-4_11"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200403022"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.76.071905.090048"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008\u20108749(03)00127\u20108"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2018.11.002"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.17717"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PGEN.1008373"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2007.08.007"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1109263108"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/eru359"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms10041911"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.BBABIO.2006.03.014"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/8060949"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.111.087403"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erj151"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-580-4_8"},{"key":"e_1_2_8_44_1","volume-title":"Studies in Optics","author":"Michelson A. A.","year":"1995"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1093\/pnasnexus\/pgac225"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2015.00078"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2015.00883"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1105\/TPC.16.00700"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0139331"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u2010313X.2010.04389.x"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1074\/JBC.REV120.015101"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1109\/ISBI.2017.7950603"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad864"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/JXB\/ERAC284"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcm001"},{"key":"e_1_2_8_56_1","unstructured":"Royle S. J.2024.\u201cTrackMateR: Working with TrackMate Outputs in R.\u201dhttps:\/\/quantixed.github.io\/TrackMateR\/."},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.108.126953"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.12394"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u2010313X.2005.02561.x"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2003.11.009"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/ern114"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1109\/ISBI52829.2022.9761696"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1266\/ggs.81.215"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2016.09.016"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2015.06.017"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg130"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601963"},{"key":"e_1_2_8_68_1","unstructured":"Wagner T.2016.\u201cij\u2010trackmate\u2010findmaxima: FindMaxima\u20101.0.1 (Version FindMaxima\u20101.0.1) [Computer software].\u201dZenodo.https:\/\/doi.org\/10.5281\/zenodo.59166."},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003\u2010020\u20100872\u2010x"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.3389\/FCELL.2021.757305\/BIBTEX"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.13400"},{"key":"e_1_2_8_72_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.97"},{"key":"e_1_2_8_73_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425\u2010009\u20101086\u20100"},{"key":"e_1_2_8_74_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gnh101"}],"container-title":["Physiologia Plantarum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ppl.70404","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ppl.70404","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ppl.70404","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ppl.70404","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T15:48:28Z","timestamp":1772984908000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ppl.70404"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":73,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1111\/ppl.70404"],"URL":"https:\/\/doi.org\/10.1111\/ppl.70404","archive":["Portico"],"relation":{},"ISSN":["0031-9317","1399-3054"],"issn-type":[{"value":"0031-9317","type":"print"},{"value":"1399-3054","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"2025-03-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70404"}}