{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T16:02:12Z","timestamp":1792252932377,"version":"4.4.0"},"reference-count":94,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T00:00:00Z","timestamp":1753142400000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Volatile organic compounds (VOCs) are produced by grapevine leaves in response to phytopathogen infection. In particular, 2\u2010phenylethanol and \u03b2\u2010cyclocitral were triggered by\n                    <jats:italic>Plasmopara viticola<\/jats:italic>\n                    inoculation in downy mildew\u2010resistant genotypes, but no information is available on their involvement in plant resistance induction. This study aimed to clarify transcriptional and metabolic changes associated with VOC\u2010induced resistance activated by 2\u2010phenylethanol and \u03b2\u2010cyclocitral treatments against\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. viticola<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Both VOCs decreased downy mildew severity on grapevine leaf disks by complex reprogramming of the grapevine transcriptome at 1 and 6\u2009days post inoculation. RNA\u2010Seq analysis showed the modulation of transcripts related to defense (chitinases, defense\u2010related proteins, and pathogenesis\u2010related proteins), oxidative stress (glutathione S\u2010transferases and peroxidases), secondary metabolism (nitrilases, stilbene synthases, and terpene synthases), signal transduction (e.g., kinases, receptor kinases, and calmodulins), and transcription (bHLH, ERF, MYB, NAC, and WRKY transcription factors) in leaf disks treated with 2\u2010phenylethanol and \u03b2\u2010cyclocitral. VOC treatments also caused changes in the leaf metabolome, and pathway analysis of metabolic features with significant changes in abundance in 2\u2010phenylethanol\u2010 and \u03b2\u2010cyclocitral\u2010treated leaf disks revealed the reprogramming of amino acid, carbohydrate, flavonoid, phenylpropanoid, and terpenoid metabolism. In particular, compounds with increases in abundance belonged to putative carbohydrates, carboxylic acids, and phenylpropanoids in 2\u2010phenylethanol\u2010treated samples, or carboxylic acids and terpenoids in \u03b2\u2010cyclocitral\u2010treated samples, including molecules possibly associated with plant defense against pathogens, such as 3\u2010dehydroquinic acid, 4\u2010thujanol, aromadendrin, camphor,\n                    <jats:italic>p<\/jats:italic>\n                    \u2010coumaryl alcohol, and perillaldehyde. Correlation analysis between transcriptomic and metabolomic data indicated that 2\u2010phenylethanol and \u03b2\u2010cyclocitral act as resistance inducers against downy mildew in grapevine.\n                  <\/jats:p>","DOI":"10.1111\/ppl.70412","type":"journal-article","created":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T03:55:02Z","timestamp":1753242902000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Volatile 2\u2010Phenylethanol and \u03b2\u2010Cyclocitral Trigger Defense\u2010Related Transcriptional and Metabolic Changes in\u00a0Grapevine Leaves Against Downy Mildew"],"prefix":"10.1111","volume":"177","author":[{"given":"Sara","family":"Avesani","sequence":"first","affiliation":[{"name":"Center for Agriculture Food Environment (C3A) University of Trento  San Michele all\u2019Adige Italy"},{"name":"Laboratory for Flavours and Metabolites Laimburg Research Centre  Auer (Ora) Italy"},{"name":"Research and Innovation Centre Fondazione Edmund Mach  San Michele all\u2019Adige Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Lazazzara","sequence":"additional","affiliation":[{"name":"Research and Innovation Centre Fondazione Edmund Mach  San Michele all\u2019Adige Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matteo","family":"Buti","sequence":"additional","affiliation":[{"name":"Department of Agriculture, Food, Environmental and Forestry Sciences (DAGRI) University of Florence  Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Oberhuber","sequence":"additional","affiliation":[{"name":"Laboratory for Flavours and Metabolites Laimburg Research Centre  Auer (Ora) Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Robatscher","sequence":"additional","affiliation":[{"name":"Laboratory for Flavours and Metabolites Laimburg Research Centre  Auer (Ora) Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7218-9963","authenticated-orcid":false,"given":"Michele","family":"Perazzolli","sequence":"additional","affiliation":[{"name":"Center for Agriculture Food Environment (C3A) University of Trento  San Michele all\u2019Adige Italy"},{"name":"Research and Innovation Centre Fondazione Edmund Mach  San Michele all\u2019Adige Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aan0826"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-023-04105-3"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2017.00101"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/eru517"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2012.04.014"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cpb.2023.100298"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppl.13771"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppa.12089"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erab520"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2020.00121"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-69051-6"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2017.01524"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2021.693887"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2019.08.003"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo13030329"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants10112465"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13321-016-0174-y"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.12145"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2338.2001.tb01000.x"},{"key":"e_1_2_10_22_1","first-page":"265","article-title":"European and Mediterranean Plant Protection Organization. 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