{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T16:29:18Z","timestamp":1791563358706,"version":"4.3.4"},"reference-count":66,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,8,27]],"date-time":"2025-08-27T00:00:00Z","timestamp":1756252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,8,27]],"date-time":"2025-08-27T00:00:00Z","timestamp":1756252800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004955","name":"\u00d6sterreichische Forschungsf\u00f6rderungsgesellschaft","doi-asserted-by":"publisher","award":["41863779"],"award-info":[{"award-number":["41863779"]}],"id":[{"id":"10.13039\/501100004955","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Light and inorganic carbon (C\n                    <jats:sub>i<\/jats:sub>\n                    ) drive photosynthesis, which fuels cellular maintenance, energy storage, and growth in photosynthetic organisms. Despite its pivotal role, how primary metabolism adjusts to contrasting light and C\n                    <jats:sub>i<\/jats:sub>\n                    availability in algae remains elusive. Here, we characterized bioenergetics and profiled primary metabolites of photoautotrophic\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Chlamydomonas reinhardtii<\/jats:italic>\n                    <\/jats:styled-content>\n                    cultures grown under constant low\/sub\u2010saturating (LL) or high\/saturating (HL) light with 2% (CO\n                    <jats:sub>2<\/jats:sub>\n                    ) or ambient 0.04% (Amb) CO\n                    <jats:sub>2<\/jats:sub>\n                    . HL\u2010Amb cells suffered photoinhibition and limitation of photosystem I electron flow at the donor side, but not the acceptor side, indicating use of alternative electron pathways to fuel ATP synthesis. Further, more glycolate was excreted under HL\u2010Amb, indicative of photorespiration. In contrast, HL\u2010CO\n                    <jats:sub>2<\/jats:sub>\n                    cells upregulated the cytochrome\n                    <jats:italic>b<\/jats:italic>\n                    <jats:sub>6<\/jats:sub>\n                    <jats:italic>f<\/jats:italic>\n                    complex, ascorbate metabolism, and PTOX2 for maintaining plastid redox homeostasis. Enhanced glycerol excretion under HL enabled dissipation of excess reducing equivalents to adjust the cellular energy balance. CO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010enhanced photosynthesis promoted respiration and primary metabolite accumulation, driving faster growth while promoting nitrogen (N) metabolism. Hence, C\n                    <jats:sub>i<\/jats:sub>\n                    \u2010dependent photoacclimation influenced the interplay between the TCA cycle and N assimilation, as supported by proteomic data. Overall, abundant C\n                    <jats:sub>i<\/jats:sub>\n                    supported growth by promoting electron flow for C\n                    <jats:sub>i<\/jats:sub>\n                    assimilation, which supplied C skeletons for N assimilation while mitigating photorespiration and photoinhibition.\n                  <\/jats:p>","DOI":"10.1111\/ppl.70461","type":"journal-article","created":{"date-parts":[[2025,8,27]],"date-time":"2025-08-27T10:40:13Z","timestamp":1756291213000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>\n                      CO\n                      <sub>2<\/sub>\n                    <\/scp>\n                    \u2010Dependent Promotion of Photosynthesis Drives Metabolic Photoacclimation in\n                    <scp>\n                      <i>Chlamydomonas reinhardtii<\/i>\n                    <\/scp>"],"prefix":"10.1111","volume":"177","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4624-6877","authenticated-orcid":false,"given":"Ana","family":"Pfleger","sequence":"first","affiliation":[{"name":"Department of Botany University of Innsbruck  Innsbruck Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2344-1426","authenticated-orcid":false,"given":"Erwann","family":"Arc","sequence":"additional","affiliation":[{"name":"Department of Botany University of Innsbruck  Innsbruck Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0259-0468","authenticated-orcid":false,"given":"Thomas","family":"Roach","sequence":"additional","affiliation":[{"name":"Department of Botany University of Innsbruck  Innsbruck Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0033583502003839"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.010405"},{"issue":"1","key":"e_1_2_9_4_1","first-page":"D609","article-title":"UniProt: The Universal Rotein Knowledgebase in 2025","volume":"52","author":"Bateman A.","year":"2025","journal-title":"Nucleic Acids Research"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(95)00298-N"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1200165"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000577"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.304279"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.19328"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.112.2.677"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-60525-7"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2021.11.007"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.12071"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.algal.2016.11.017"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3054.2003.00078.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/plcell\/koae203"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.12825"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.66.4.592"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5687(01)00307-3"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470372531.ch12"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw313"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2018.01265"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/plcell\/koad157"},{"key":"e_1_2_9_24_1","first-page":"69","volume-title":"Plant\u2013Environment Interactions","author":"Humby P. L.","year":"2006"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pp.45.060194.003045"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpy.13417"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00318-12"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1618922114"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01089043"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-3002(56)90003-8"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00294-008-0224-8"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erh237"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2011.04537.x"},{"issue":"7","key":"e_1_2_9_34_1","article-title":"High Quality Metabolomic Data for Chlamydomonas reinhardtii","volume":"4","author":"Lee D. Y.","year":"2008","journal-title":"Plant Methods"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/md17010001"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcw129"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.08.044"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118567166.ch2"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.13530"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.82.3.821"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.1995.tb00967.x"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02598"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.25.14162"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08587"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11120-010-9575-3"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2024.149497"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1101\/2025.05.19.654497"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.16.01310"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3040.2003.01004.x"},{"key":"e_1_2_9_50_1","volume-title":"A Language and Environment for Statistical Computing","author":"R Core Team","year":"2024"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv007"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppl.12567"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.12768"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/eraa022"},{"key":"e_1_2_9_55_1","first-page":"15","article-title":"Non\u2010Photochemical Fluorescence Quenching and Quantum Yields in PS I and PS II: Analysis of Heat\u2010Induced Limitations Using Maxi\u2010Imaging\u2010PAM and Dual\u2010PAM\u2010100","volume":"1","author":"Schreiber U.","year":"2008","journal-title":"PAM Application Notes"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00024185"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.13324"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.93.4.1433"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2010.05.006"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.010896"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.5.2213"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241612873"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/30728"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-24277-4"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(01)00212-2"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-48789-x"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppl.14630"}],"container-title":["Physiologia Plantarum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ppl.70461","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ppl.70461","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ppl.70461","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T10:24:48Z","timestamp":1776248688000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ppl.70461"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,27]]},"references-count":66,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1111\/ppl.70461"],"URL":"https:\/\/doi.org\/10.1111\/ppl.70461","archive":["Portico"],"relation":{},"ISSN":["0031-9317","1399-3054"],"issn-type":[{"value":"0031-9317","type":"print"},{"value":"1399-3054","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,27]]},"assertion":[{"value":"2025-06-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70461"}}