{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T11:09:27Z","timestamp":1776251367405,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,8,27]],"date-time":"2025-08-27T00:00:00Z","timestamp":1756252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,8,27]],"date-time":"2025-08-27T00:00:00Z","timestamp":1756252800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100017691","name":"Guangxi Key Research and Development Program","doi-asserted-by":"publisher","award":["AB23026086"],"award-info":[{"award-number":["AB23026086"]}],"id":[{"id":"10.13039\/501100017691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Theobromine is a significant purine alkaloid found in tea plants, known for its various bioactive functions in humans. While the metabolic pathway for purine alkaloids, particularly involving the methyltransferase CsTCS1, has been largely understood, information regarding the upstream regulatory network of theobromine remains limited. In this study, we collected transcriptome data from tea plants exhibiting different levels of purine alkaloids and applied weighted gene co\u2010expression network analysis (WGCNA) to identify the upstream regulatory network modules associated with each purine alkaloid. The module labeled MEred was discovered as one of the negative regulatory network modules affecting theobromine, with the gene\n                    <jats:italic>CsMADS<\/jats:italic>\n                    identified as a hub gene within this module. To investigate the role of CsMADS in theobromine accumulation, we examined the concentrations of purine alkaloids and the expression patterns of related genes in the\n                    <jats:italic>CsMADS<\/jats:italic>\n                    \u2010silenced tea plants. Results showed that theobromine levels increased in the tender leaves of\n                    <jats:italic>CsMADS<\/jats:italic>\n                    \u2010silenced tea plants, while the expression level of\n                    <jats:italic>CsTCS1<\/jats:italic>\n                    decreased in these plants. Meanwhile, CsMADS did not influence mechanical harvesting\u2010related traits such as leaf droopiness or leaf tip expansion. In summary, CsMADS negatively regulates the theobromine content in tea leaves without adversely affecting mechanical harvesting capabilities.\n                  <\/jats:p>","DOI":"10.1111\/ppl.70479","type":"journal-article","created":{"date-parts":[[2025,8,27]],"date-time":"2025-08-27T10:59:25Z","timestamp":1756292365000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>CsMADS<\/scp>\n                    Negatively Regulates the Theobromine Content of Leaves in Tea Plants"],"prefix":"10.1111","volume":"177","author":[{"given":"Lingxiao","family":"Duan","sequence":"first","affiliation":[{"name":"State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization Tea Research Institute of the Chinese Academy of Agricultural Sciences  Hangzhou China"},{"name":"Yunnan Key Laboratory of Tea Germplasm Conservation and Utilization in the Lancang River Basin West Yunnan University  Lincang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanmo","family":"Fang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization Tea Research Institute of the Chinese Academy of Agricultural Sciences  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianqiang","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization Tea Research Institute of the Chinese Academy of Agricultural Sciences  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengcheng","family":"Yuan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization Tea Research Institute of the Chinese Academy of Agricultural Sciences  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiqiang","family":"Jin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization Tea Research Institute of the Chinese Academy of Agricultural Sciences  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weizhong","family":"He","sequence":"additional","affiliation":[{"name":"Lishui Institute of Agriculture and Forestry Sciences  Lishui China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7570-4389","authenticated-orcid":false,"given":"Haoran","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization Tea Research Institute of the Chinese Academy of Agricultural Sciences  Hangzhou China"},{"name":"Yunnan Key Laboratory of Tea Germplasm Conservation and Utilization in the Lancang River Basin West Yunnan University  Lincang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization Tea Research Institute of the Chinese Academy of Agricultural Sciences  Hangzhou China"},{"name":"Yunnan Key Laboratory of Tea Germplasm Conservation and Utilization in the Lancang River Basin West Yunnan University  Lincang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-408061-4.00004-3"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-nutr-071811-150642"},{"key":"e_1_2_9_4_1","first-page":"423","article-title":"Researches on the Biochemical Characteristics of Chaozhou Wild Tea Plants Which Containing Theobromine Mainly","volume":"49","author":"Huang Y.","year":"2022","journal-title":"Journal of Tea Communication"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2016.04.032"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.3c07736"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/35023072"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2011.641041"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.13814"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/hr\/uhae074"},{"key":"e_1_2_9_11_1","first-page":"29","article-title":"The Genetic Variation of the Chemical Components of the \u2018Jinxuan\u2019 \u00d7 \u2018Zijuan\u2019 F1 Segregating Population Based on UPLC","volume":"1","author":"Liu Q.","year":"2022","journal-title":"Journal of Tea Science"},{"key":"e_1_2_9_12_1","first-page":"41","article-title":"The Screening and Identification of Key Transcription Factor Genes for Theacrine Metabolism","volume":"42","author":"Liu Y.","year":"2022","journal-title":"Journal of Tea Science"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.8b05355"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/hr\/uhab046"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2021.01.001"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-011-9785-x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201600670"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/ed042p675"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(72)80815-9"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/hr\/uhad162"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.48130\/BPR-2022-0013"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.1c06886"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/hr\/uhac191"}],"container-title":["Physiologia Plantarum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ppl.70479","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ppl.70479","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ppl.70479","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T10:26:38Z","timestamp":1776248798000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ppl.70479"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,27]]},"references-count":22,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1111\/ppl.70479"],"URL":"https:\/\/doi.org\/10.1111\/ppl.70479","archive":["Portico"],"relation":{},"ISSN":["0031-9317","1399-3054"],"issn-type":[{"value":"0031-9317","type":"print"},{"value":"1399-3054","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,27]]},"assertion":[{"value":"2025-06-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70479"}}