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However, the molecular mechanism underlying NO\u2010induced plant immunity remains largely elusive. Here, we investigated the dynamics of NO biosynthesis and its downstream signaling through\n                    <jats:italic>S\u2010<\/jats:italic>\n                    nitrosoproteome analysis of two rice cultivars, Dongjin (DJ, resistant) and Nipponbare (NIP, susceptible), in response to\n                    <jats:italic>Magnaporthe oryzae<\/jats:italic>\n                    , the causative agent of the rice blast disease.\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>M. oryzae<\/jats:italic>\n                    <\/jats:styled-content>\n                    inoculation triggered relatively higher nitric oxide synthase (NOS\/NOA1)\u2010mediated NO biosynthesis in DJ than that of NIP. High\u2010throughput, site\u2010specific\n                    <jats:italic>S\u2010<\/jats:italic>\n                    nitrosoproteome analysis using the iodoTMT\u2010based mass spectrometry approach led to the identification of 511\n                    <jats:italic>S\u2010<\/jats:italic>\n                    nitrosated peptides corresponding to 335 proteins, representing the most comprehensive set of\n                    <jats:italic>S\u2010<\/jats:italic>\n                    nitrosated peptides identified in rice so far. In particular, the\n                    <jats:italic>S\u2010<\/jats:italic>\n                    nitrosated site intensity of superoxide dismutase (SOD) at Cysteine\n                    <jats:sup>263<\/jats:sup>\n                    was significantly reduced specifically in DJ in response to pathogen inoculation. We observed that in\u00a0vitro\n                    <jats:italic>S\u2010<\/jats:italic>\n                    nitrosation of SOD enhances its activity, and its\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>M. oryzae<\/jats:italic>\n                    <\/jats:styled-content>\n                    infection\u2010triggered denitrosation was correlated with the\n                    <jats:italic>S\u2010<\/jats:italic>\n                    nitrosoglutathione reductase (GSNOR) activation. This denitrosation\u2010mediated suppression of SOD activity likely leads to the accumulation of superoxide ions during infection, which triggers immune responses. Altogether, our results suggest that NO\u2010mediated\n                    <jats:italic>S\u2010<\/jats:italic>\n                    nitrosation of SOD plays a crucial role in orchestrating redox\u2010dependent defense signaling, which likely contributes to the contrasting resistance responses observed in the two cultivars. These findings provide novel insights into the functional implications of\n                    <jats:italic>S\u2010<\/jats:italic>\n                    nitrosation in plant immunity and highlight redox\u2010regulatory proteins as key targets of NO signaling during pathogen challenge.\n                  <\/jats:p>","DOI":"10.1111\/ppl.70515","type":"journal-article","created":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T02:23:54Z","timestamp":1758507834000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["<scp>\n                      <i>S<\/i>\n                    <\/scp>\n                    \u2010Nitrosoproteome Analysis of Rice Leaves Highlights the Possible Roles of Superoxide Dismutase in Resistance Against\n                    <scp>\n                      <i>Magnaporthe Oryzae<\/i>\n                    <\/scp>"],"prefix":"10.1111","volume":"177","author":[{"given":"Adeela","family":"Munir","sequence":"first","affiliation":[{"name":"Department of Plant Bioscience, Life and Industry Convergence Research Institute Pusan National University  Miryang Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheol Woo","family":"Min","sequence":"additional","affiliation":[{"name":"Department of Plant Bioscience, Life and Industry Convergence Research Institute Pusan National University  Miryang Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiming","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education Nanjing Agricultural University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9705-6868","authenticated-orcid":false,"given":"Sun Tae","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Plant Bioscience, Life and Industry Convergence Research Institute Pusan National University  Miryang Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5242-9528","authenticated-orcid":false,"given":"Ravi","family":"Gupta","sequence":"additional","affiliation":[{"name":"Plant Stress Physiology and Proteomics Laboratory, College of General Education Kookmin University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200800985"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/15592324.2015.1071753"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.4161\/psb.5.7.11899"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcn196"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty1058"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313x.2000.00911.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/152308603321223522"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/29087"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.231178298"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-020518-115810"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/32.1.79"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/15592324.2014.998544"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppl.70180"},{"key":"e_1_2_9_15_1","volume-title":"Plant Mitochondria. 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