{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T11:09:20Z","timestamp":1776251360703,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T00:00:00Z","timestamp":1760832000000},"content-version":"vor","delay-in-days":48,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82173934"],"award-info":[{"award-number":["82173934"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005090","name":"Beijing Nova Program","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005090","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Shikonin derivatives from\n                    <jats:italic>Arnebia euchroma<\/jats:italic>\n                    (Royle) Johnst. roots possess significant economic value as pharmaceutical agents, natural dyes, and cosmetic additives. To investigate hypoxia's impact on shikonin biosynthesis and its mechanisms, we subjected\n                    <jats:italic>A. euchroma<\/jats:italic>\n                    roots to hypoxic stress (1% O\n                    <jats:sub>2<\/jats:sub>\n                    ). Hypoxic roots remained white with minimal shikonin accumulation. Upon reoxygenation (\u224820% O\n                    <jats:sub>2<\/jats:sub>\n                    ), rapid shikonin synthesis occurred within 4\u2009h. qRT\u2010PCR revealed suppressed expression of shikonin pathway genes (\n                    <jats:italic>CYP76B74<\/jats:italic>\n                    ,\n                    <jats:italic>CYP76B100<\/jats:italic>\n                    ,\n                    <jats:italic>AePGT<\/jats:italic>\n                    , etc.) under hypoxia. Transcriptome analysis of roots at 0\u2009h (hypoxia), 4\u2009h, and 48\u2009h (reoxygenation) showed differential gene enrichment in carbon metabolism, phenylpropanoid biosynthesis, and glycolysis. Aerobic respiration genes were upregulated post\u2010reoxygenation, while anaerobic respiration genes declined. Critically, genes regulating upstream shikonin pathways (MVA\/MEP\u2010PP) were inhibited under hypoxia. These results demonstrate hypoxia's transcriptional repression of shikonin biosynthesis and identify oxygen\u2010dependent metabolic switches limiting production. These genes are involved in regulating the upstream MVA\/MEP\u2010PP pathway of shikonin biosynthesis, further confirming the inhibitory effect of hypoxia on shikonin biosynthesis.\n                  <\/jats:p>","DOI":"10.1111\/ppl.70591","type":"journal-article","created":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T01:16:14Z","timestamp":1760922974000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of Hypoxia on Shikonin Biosynthesis of\n                    <i>Arnebia euchroma<\/i>\n                    Roots and Its Transcriptome Analysis"],"prefix":"10.1111","volume":"177","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-3275-8309","authenticated-orcid":false,"given":"Qi","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory for Quality Ensurance and Sustainable use of Dao\u2010di Herbs National Resource Center for Chinese Materia Medica China Academy of Chinese Medical 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