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However, the precise role of the\n Ii<\/jats:italic>\n WRKY34 transcriptional network in modulating root growth remains to be elucidated. Here, we observed that heterogeneous expression of\n Ii<\/jats:italic>\n WRKY34 increased the primary root length in\n \n Arabidopsis thaliana<\/jats:italic>\n <\/jats:styled-content>\n .\n Ii<\/jats:italic>\n WRKY34 bound directly to the promoter of\n Ii<\/jats:italic>\n NAC1, which itself bound to the promoter of an auxin biosynthesis gene,\n Ii<\/jats:italic>\n YUCCA6. Molecular docking displayed the complex of\n Ii<\/jats:italic>\n WRKY34\u2010DBD and the DNA duplex of W\u2010box on the promoter. Additionally, an interaction occurred between\n Ii<\/jats:italic>\n NAC1 and\n Ii<\/jats:italic>\n NAC18, and heterogeneous expression of either transcription factor resulted in the same phenotype as\n Ii<\/jats:italic>\n WRKY34\u2010expressing lines of\n \n A. thaliana<\/jats:italic>\n <\/jats:styled-content>\n . Thus, we believe that\n Ii<\/jats:italic>\n WRKY34 functions as an upstream trigger of auxin signaling during root growth. The resulting model clarifies the molecular mechanism of\n Ii<\/jats:italic>\n WRKY34\u2010regulated root growth, which may have applications in enhancing agricultural yields of\n I. indigotica<\/jats:italic>\n and possibly other plants in the family Brassicaceae.\n <\/jats:p>","DOI":"10.1111\/ppl.70631","type":"journal-article","created":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T04:14:57Z","timestamp":1762920897000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["\n Ii<\/i>\n WRKY34\n <\/scp>\n Regulates Plant Root Growth Through a Direct Interaction With\n NAC1<\/scp>\n and Its Interactor\n NAC18<\/scp>\n in\n \n Isatis indigotica<\/i>\n <\/scp>"],"prefix":"10.1111","volume":"177","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-8819-7387","authenticated-orcid":false,"given":"Jingxian","family":"Feng","sequence":"first","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of 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