{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T09:49:55Z","timestamp":1782294595418,"version":"3.54.5"},"reference-count":58,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T00:00:00Z","timestamp":1781136000000},"content-version":"vor","delay-in-days":41,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Styphnolobium japonicum<\/jats:italic>\n                    <\/jats:styled-content>\n                    is valued for its flavonoid\u2010rich flowers, yet the developmental regulation of these metabolites remains unclear. Here, we integrated transcriptomic and metabolomic analyzes across four floral stages to reveal dynamic changes in flavonoid biosynthesis. Flavonoids emerged as the predominant metabolites, showing distinct stage\u2010specific accumulation. Early stages showed high expression of\n                    <jats:italic>CHS<\/jats:italic>\n                    and\n                    <jats:italic>F3H<\/jats:italic>\n                    , with naringenin and eriodictyol as dominant metabolites. Mid\u2010development maintained\n                    <jats:italic>FLS<\/jats:italic>\n                    \u2010driven flavonol production, yielding quercetin and kaempferol derivatives. Late stages exhibited coordinated upregulation of\n                    <jats:italic>CYP93C<\/jats:italic>\n                    and\n                    <jats:italic>HIDH<\/jats:italic>\n                    , driving isoflavone (genistein) enrichment, alongside\n                    <jats:italic>ANS<\/jats:italic>\n                    and\n                    <jats:italic>F3GT<\/jats:italic>\n                    upregulation, enhancing anthocyanin glycosylation. Weighted gene co\u2010expression and KEGG enrichment analyzes further supported a temporal reprogramming from primary metabolism to specialized bioactive compound production. Coordinated gene\u2013metabolite patterns highlighted a temporal reprogramming from primary metabolism to targeted bioactive compound production. This study provides molecular insight into flavonoid biosynthesis during floral development and offers a framework for metabolic enhancement in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. japonicum<\/jats:italic>\n                    <\/jats:styled-content>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/ppl.70967","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T04:01:36Z","timestamp":1781236896000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Transcriptomic and Metabolomic Profiling Reveals Developmental Reprogramming of Flavonoid Biosynthesis in\n                    <i>Styphnolobium japonicum<\/i>\n                    Flowers"],"prefix":"10.1111","volume":"178","author":[{"given":"Huiyuan","family":"Liu","sequence":"first","affiliation":[{"name":"College of Life Sciences and Engineering, Henan University of Urban Construction  Pingdingshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Henan Engineering Research Center for Osmanthus Germplasm Innovation and Resource Utilization Henan Agricultural University  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6074-742X","authenticated-orcid":false,"given":"Miao","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Engineering, Henan University of Urban Construction  Pingdingshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Guo","sequence":"additional","affiliation":[{"name":"Henan Engineering Research Center for Osmanthus Germplasm Innovation and Resource Utilization Henan Agricultural University  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yihan","family":"Wang","sequence":"additional","affiliation":[{"name":"Henan Engineering Research Center for Osmanthus Germplasm Innovation and Resource Utilization Henan Agricultural University  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaohui","family":"Xie","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Engineering, Henan University of Urban Construction  Pingdingshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijie","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Engineering, Henan University of Urban Construction  Pingdingshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fude","family":"Shang","sequence":"additional","affiliation":[{"name":"Henan Engineering Research Center for Osmanthus Germplasm Innovation and Resource Utilization Henan Agricultural University  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4002-0271","authenticated-orcid":false,"given":"Hongwei","family":"Wang","sequence":"additional","affiliation":[{"name":"Henan Engineering Research Center for Osmanthus Germplasm Innovation and Resource Utilization Henan Agricultural University  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,11]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00044-014-1101-2"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbl016"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2024.108978"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.19096"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0026683"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40643-024-00825-w"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.4c11116"},{"key":"e_1_2_12_9_1","article-title":"Flavonoids: Biosynthesis, Biological Functions, and Biotechnological Applications","volume":"3","author":"Ferreyra M. 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