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Traditional transcriptome sequencing can only perform mixed\u2010sequencing analysis on multicellular tissues and fails to detect intercellular heterogeneity. However, the groundbreaking development and integration of single\u2010cell RNA sequencing (scRNA\u2010seq) and spatial transcriptomics (ST) are leading forest tree biology into a new era. This review systematically illustrates the developmental history, technical workflows, and integration of these two technologies and elaborates in detail on their cutting\u2010edge applications in woody plants, such as vascular cambium differentiation, abiotic stress response analysis, and the identification and characterization of rare cell types. In addition, we discuss the challenges encountered in applying these technologies to forest tree research and point out the key directions for future research that should be focused on solving the difficulties in upcoming woody plant studies. 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