{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T09:13:39Z","timestamp":1788426819265,"version":"build-2803163510"},"reference-count":51,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":41,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32460743"],"award-info":[{"award-number":["32460743"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100019534","name":"Science Fund for Distinguished Young Scholars of Guangxi Province","doi-asserted-by":"publisher","award":["2025GXNSFFA069005"],"award-info":[{"award-number":["2025GXNSFFA069005"]}],"id":[{"id":"10.13039\/501100019534","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study uncovers a novel concentration\u2010dependent signaling network through which abscisic acid (ABA) modulates acid metabolism in grape berries. Treatment with 800\u2009\u03bcM ABA significantly decreased malate and citrate synthesis and transport while enhancing glucose and fructose accumulation, optimizing the sugar\u2010acid ratio. Multi\u2010omics analysis revealed differential changes in 96 organic acids and identified a key malate transporter, VvALMT9. Through yeast two\u2010hybrid screening, the VvPP2C\u2010VvALMT9\u2010VvSnRK2.3\u2010VvCDPK interaction network was characterized in a dual regulatory mode. Under low\u2010ABA conditions, VvSnRK2.3 and VvPP2C are synergistically upregulated to impair VvALMT9 function via direct binding and transcriptional repression, respectively, while reduced\n                    <jats:italic>VvCDPK<\/jats:italic>\n                    expression leads to insufficient VvSnRK2.3 phosphorylation, establishing a \u201clow ABA\u2010high VvALMT9\u201d state with high acid accumulation; when low temperature is coupled with low ABA, the inactivation of the temperature\u2010sensing domain of VvPP2C further relieves this repression, delaying acid degradation. Conversely, a high\u2010ABA environment suppresses the basal expression of\n                    <jats:italic>VvPP2C<\/jats:italic>\n                    \/\n                    <jats:italic>VvSnRK2.3<\/jats:italic>\n                    and activates the VvCDPK\u2010VvSnRK2.3 cascade to efficiently inhibit ALMT9 phosphorylation, blocking malate accumulation and reducing acidity. Transgenic validation demonstrated that\n                    <jats:italic>VvALMT9<\/jats:italic>\n                    overexpression promotes acid accumulation under ambient temperature, whereas high temperature induces\n                    <jats:italic>VvPP2C<\/jats:italic>\n                    to inhibit VvALMT9, an effect reversed by ABA. Overall, this study elucidates the mechanism of ABA\u2010temperature synergistic regulation of VvALMT9\u2010mediated acid metabolism homeostasis, providing a theoretical basis for targeted fruit quality improvement.\n                  <\/jats:p>","DOI":"10.1111\/ppl.71030","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T12:08:12Z","timestamp":1786450092000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Abscisic Acid Lowers Postharvest Grape Acidity via\n                    <scp>PP2C<\/scp>\n                    \u2013\n                    <scp>ALMT9<\/scp>\n                    \u2010Dependent Malate Transport"],"prefix":"10.1111","volume":"178","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-4608-5339","authenticated-orcid":false,"given":"Yunzhi","family":"Zhou","sequence":"first","affiliation":[{"name":"State Key Laboratory for Conservation and Utilization of Subtropical 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